製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Aquaporin 5
  • アプリケーション
    適用あり: IHC-P, ELISA, WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Dog
  • 免疫原

    A 17 amino acid synthetic peptide corresponding to the carboxy terminus of rat Aquaporin 5

  • ポジティブ・コントロール
    • Rat salivary gland lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab78486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2 - 10 µg/ml.
ELISA Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.05 - 0.1 µg/ml. Predicted molecular weight: 29 kDa.
IHC-Fr Use at an assay dependent concentration. PubMed: 21704616

ターゲット情報

  • 機能
    Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Required for TRPV4 activation by hypotonicity (PubMed:16571723). Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723).
  • 関連疾患
    Keratoderma, palmoplantar, Bothnian type
  • 配列類似性
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • ドメイン
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • 細胞内局在
    Apical cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AQP 5 antibody
    • AQP-5 antibody
    • AQP5 antibody
    • AQP5_HUMAN antibody
    • Aquaporin 5 antibody
    • Aquaporin-5 antibody
    see all

画像

  • Lane 1 : Anti-Aquaporin 5 antibody (ab78486) at 0.05 µg/ml
    Lane 2 : Anti-Aquaporin 5 antibody (ab78486) at 0.1 µg/ml

    Lane 1 : rat submaxillary (salivary) gland lysate
    Lane 2 : rat submaxillary (salivary) gland lysate


    Predicted band size : 29 kDa
    Observed band size : 29 kDa

参考文献

This product has been referenced in:
  • Tong L  et al. Fibroblast Growth Factor-10 (FGF-10) Mobilizes Lung-resident Mesenchymal Stem Cells and Protects Against Acute Lung Injury. Sci Rep 6:21642 (2016). IF ; Rat . Read more (PubMed: 26869337) »
  • Cao Z  et al. Targeting of the pulmonary capillary vascular niche promotes lung alveolar repair and ameliorates fibrosis. Nat Med 22:154-62 (2016). Read more (PubMed: 26779814) »

See all 16 Publications for this product

レビューと Q&A

Application
CyTOF
Sample
Mouse Cell (Lung)
Specification
Lung
Username

Irit Milman

Verified customer

投稿 Nov 14 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris NuPAGE)
Loading amount
40 µg
Specification
Lung
Blocking step
Odyssey TBS Blocking Buffer (proprietary) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Pete Serbedzija

Verified customer

投稿 May 25 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (HEK cells transfected with AQP5-EGFP)
Permeabilization
Yes - 0,2% Saponin
Specification
HEK cells transfected with AQP5-EGFP
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 03 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (primarly lung epithelia cells)
Specification
primarly lung epithelia cells
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 0.2% · Temperature: 21°C
Fixative
Methanol
Username

Abcam user community

Verified customer

投稿 Oct 08 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Enzymatic
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 20 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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