Anti-Aquaporin 4 抗体 (ab81355)

製品の概要

  • 製品名Anti-Aquaporin 4 antibody
    Aquaporin 4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Aquaporin 4
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    A synthetic peptide corresponding to a sequence at the C-terminal of Human Aquaporin 4

  • ポジティブ・コントロール
    • ICC/IF: SKNSH cells.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab81355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.Can be blocked with Aquaporin 4 peptide (ab181722).

ターゲット情報

  • 機能Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
  • 組織特異性Brain - muscle >> heart, kidney, lung, and trachea.
  • 配列類似性Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • ドメインAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • 翻訳後修飾Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40%.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AQP 4 antibody
    • AQP-4 antibody
    • AQP4 antibody
    • AQP4_HUMAN antibody
    • Aquaporin type 4 antibody
    • Aquaporin-4 antibody
    • Aquaporin4 antibody
    • HMIWC 2 antibody
    • HMIWC2 antibody
    • Mercurial insensitive water channel antibody
    • Mercurial-insensitive water channel antibody
    • MGC22454 antibody
    • MIWC antibody
    • WCH 4 antibody
    • WCH4 antibody
    see all

Anti-Aquaporin 4 antibody 画像

  • ICC/IF image of ab81355 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81355, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Aquaporin 4 antibody (ab81355) at 1 µg/ml

    Lane 1 : Tissue lysates prepared from Rat heart tissue.
    Lane 2 : Tissue lysates prepared from Rat kidney tissue.
    Lane 3 : Tissue lysates prepared from Rat brain tissue.
    Lane 4 : Tissue lysates prepared from Rat brain tissue.

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution

    Predicted band size : 35 kDa

Anti-Aquaporin 4 antibody (ab81355) 使用論文

ab81355 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (Muscle)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 30 µg
Specification Muscle
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Username

Miss. Nathalie Da Silva

Verified customer

投稿 Jul 07 2016

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