製品の概要

  • 製品名Anti-Aquaporin 3 antibody
    Aquaporin 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Aquaporin 3
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Rabbit, Horse
  • 免疫原

    Synthetic peptide:

    N-CHLEQPPPSTEAENVKLAHMKHKEQI

    , corresponding to C terminal amino acids 267-292 of Rat Aquaporin 3 (NP_113891.1).

  • ポジティブ・コントロール
    • Rat kidney tissue; Rat kidney lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human Brain Hippocampus (Alzheimer's).

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab125045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/4000. Predicted molecular weight: 31 kDa.
ICC/IF 1/200 - 1/400.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.
  • 組織特異性Widely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level.
  • 配列類似性Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • ドメインAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • 細胞内局在Basolateral cell membrane. In collecting ducts of kidney.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AQP 3 antibody
    • AQP-3 antibody
    • Aqp3 antibody
    • AQP3_HUMAN antibody
    • Aquaglyceroporin-3 antibody
    • Aquaporin 3 (GIL blood group) antibody
    • Aquaporin 3 (Gill blood group) antibody
    • Aquaporin-3 antibody
    • Aquaporin3 antibody
    • GIL antibody
    • Gill blood group antibody
    see all

Anti-Aquaporin 3 antibody 画像

  • IHC image of Aquaporin 3 staining in Human Hippocampus (Alzheimer's) formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab125045, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab125045, at 1/200 dilution, staining Aquaporin 3 in Rat kidney tissues by Immunofluorescence.
  • All lanes : Anti-Aquaporin 3 antibody (ab125045) at 1/2000 dilution

    Lane 1 : Rat kidney lysate
    Lane 2 : Rat kidney lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : Rat kidney lysate


    Predicted band size : 31 kDa

Anti-Aquaporin 3 antibody (ab125045) 使用論文

ab125045 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"