Anti-Aquaporin 2 (phospho S261) 抗体 (ab64154)
製品の概要
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製品名Anti-Aquaporin 2 (phospho S261) antibody
Aquaporin 2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Aquaporin 2 (phospho S261)
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由来種Rabbit
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特異性This antibody is specific for ~29k Aquaporin 2 protein phosphorylated at Ser261. It also recognizes the glycosylated form of Aquaporin 2 at ~ 37k. Immunolabeling of the Aquaporin 2 band is blocked by preadsorption with the phospho peptide used as antigen but not by the corresponding dephospho peptide.
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アプリケーション適用あり: WB, Flow Cytmore details
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種交差性交差種: Rat
交差が予測される動物種: Mouse, Chicken, Cow, Dog, Human, Non human primates
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免疫原
Phosphopeptide corresponding to amino acid residues surrounding the phospho Serine 261 of rat Aquaporin 2.
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ポジティブ･コントロール
- Rat kidney lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: None
Constituents: 50% Glycerol, 0.1mg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab64154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
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| WB | 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). | |
| Flow Cyt | Use at an assay dependent concentration. PubMed: 20826573 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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機能Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
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組織特異性Expressed in renal collecting tubules.
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関連疾患Diabetes insipidus, nephrogenic, autosomal
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配列類似性Belongs to the MIP/aquaporin (TC 1.A.8) family.
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ドメインAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
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翻訳後修飾Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
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細胞内局在Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated.
- Information by UniProt
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参照データベース
- Entrez Gene: 539870 Cow
- Entrez Gene: 359 Human
- Entrez Gene: 11827 Mouse
- Entrez Gene: 25386 Rat
- Omim: 107777 Human
- SwissProt: P79099 Cow
- SwissProt: P79144 Dog
- SwissProt: P41181 Human
see all -
別名
- ADH water channel antibody
- AQP 2 antibody
- AQP CD antibody
see all
画像
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Western blot - Aquaporin 2 (phospho S261) antibody (ab64154)All lanes : Anti-Aquaporin 2 (phospho S261) antibody (ab64154) at 1/1000 dilution
Lane 1 : Rat kidney lysate
Lane 2 : Rat kidney lysate, with blocking peptide
Predicted band size: 29 kDa
Observed band size: 29,37 kDa (why is the actual band size different from the predicted?)
参考文献
This product has been referenced in:
- Kelley R et al. Tubular cell-enriched subpopulation of primary renal cells improves survival and augments kidney function in rodent model of chronic kidney disease. Am J Physiol Renal Physiol 299:F1026-39 (2010). Flow Cyt ; Rat . Read more (PubMed: 20826573) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"