特異性This antibody is specific for ~29k Aquaporin 2 protein phosphorylated at Ser261. It also recognizes the glycosylated form of Aquaporin 2 at ~ 37k. Immunolabeling of the Aquaporin 2 band is blocked by preadsorption with the phospho peptide used as antigen but not by the corresponding dephospho peptide.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
Use at an assay dependent concentration. PubMed: 20826573ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
機能Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
組織特異性Expressed in renal collecting tubules.
関連疾患Diabetes insipidus, nephrogenic, autosomal
配列類似性Belongs to the MIP/aquaporin (TC 1.A.8) family.
ドメインAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
翻訳後修飾Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
細胞内局在Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated.
Kelley R et al. Tubular cell-enriched subpopulation of primary renal cells improves survival and augments kidney function in rodent model of chronic kidney disease. Am J Physiol Renal Physiol299:F1026-39 (2010).
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