製品の概要
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製品名Anti-Aquaporin 2 (phospho S256) antibody
Aquaporin 2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Aquaporin 2 (phospho S256)
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由来種Rabbit
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特異性ab111346 detects endogenous levels of Aquaporin 2 only when phosphorylated at serine 256.
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アプリケーション適用あり: IHC-Pmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic phosphopeptide phosphopeptide derived from Human Aquaporin 2 around the phosphorylation site of serine 256 (R-Q-SP-V-E).
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ポジティブ･コントロール
- Human brain tissue.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: PBS (without Mg2+ and Ca2+) -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）ab111346 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab111346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
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組織特異性Expressed in renal collecting tubules.
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関連疾患Diabetes insipidus, nephrogenic, autosomal
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配列類似性Belongs to the MIP/aquaporin (TC 1.A.8) family.
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ドメインAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
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翻訳後修飾Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
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細胞内局在Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated.
- Information by UniProt
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参照データベース
- Entrez Gene: 359 Human
- Entrez Gene: 11827 Mouse
- Entrez Gene: 25386 Rat
- Omim: 107777 Human
- SwissProt: P41181 Human
- SwissProt: P56402 Mouse
- SwissProt: P34080 Rat
- Unigene: 130730 Human
see all -
別名
- ADH water channel antibody
- AQP 2 antibody
- AQP CD antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 2 (phospho S256) antibody (ab111346)ab111346 at 1/50 dilution staining Aquaporin 2 in Paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.
参考文献
ab111346 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"