Anti-Aquaporin 2 (phospho S256) 抗体 (ab111346)
Key features and details
- Rabbit polyclonal to Aquaporin 2 (phospho S256)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Aquaporin 2 (phospho S256) antibody
Aquaporin 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Aquaporin 2 (phospho S256) -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Aquaporin 2 (phospho S256).
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ポジティブ・コントロール
- Human brain tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS (without Mg2+ and Ca2+) -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab111346 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111346の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. -
組織特異性
Expressed in renal collecting tubules. -
関連疾患
Diabetes insipidus, nephrogenic, autosomal -
配列類似性
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
ドメイン
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
翻訳後修飾
Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. -
細胞内局在
Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated. - Information by UniProt
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参照データベース
- Entrez Gene: 359 Human
- Entrez Gene: 11827 Mouse
- Entrez Gene: 25386 Rat
- Omim: 107777 Human
- SwissProt: P41181 Human
- SwissProt: P56402 Mouse
- SwissProt: P34080 Rat
- Unigene: 130730 Human
see all -
別名
- ADH water channel antibody
- AQP 2 antibody
- AQP CD antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab111346 は 4 報の論文で使用されています。
- Kim S et al. Psychotropic drugs upregulate aquaporin-2 via vasopressin-2 receptor/cAMP/protein kinase A signaling in inner medullary collecting duct cells. Am J Physiol Renal Physiol 320:F963-F971 (2021). PubMed: 33843270
- Wang R et al. Pdcd10-Stk24/25 complex controls kidney water reabsorption by regulating Aqp2 membrane targeting. JCI Insight 6:N/A (2021). PubMed: 34156031
- Kong Y et al. Statins ameliorate cholesterol-induced inflammation and improve AQP2 expression by inhibiting NLRP3 activation in the kidney. Theranostics 10:10415-10433 (2020). PubMed: 32929357
- Huang J et al. Arginase-II negatively regulates renal aquaporin-2 and water reabsorption. FASEB J 32:5520-5531 (2018). PubMed: 29718707