製品の概要

  • 製品名Anti-Aquaporin 2 antibody
    Aquaporin 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Aquaporin 2
  • 特異性Detects endogenous levels of total Aquaporin 2.
  • アプリケーション適用あり: ELISA, ICC/IF, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human Aquaporin 2 around the phosphorylation site of serine 256

  • ポジティブ・コントロール
    • Hela cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab62628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
IHC-Fr 1/500.

ターゲット情報

  • 機能Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • 組織特異性Expressed in renal collecting tubules.
  • 関連疾患Diabetes insipidus, nephrogenic, autosomal
  • 配列類似性Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • ドメインAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • 翻訳後修飾Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
  • 細胞内局在Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADH water channel antibody
    • AQP 2 antibody
    • AQP CD antibody
    • AQP-2 antibody
    • AQP-CD antibody
    • AQP2 antibody
    • AQP2_HUMAN antibody
    • AQPCD antibody
    • Aquaporin 2 collecting duct antibody
    • Aquaporin CD antibody
    • Aquaporin-2 antibody
    • Aquaporin-CD antibody
    • Aquaporin2 antibody
    • Aquaporine 2 antibody
    • Collecting duct water channel protein antibody
    • MGC34501 antibody
    • Water channel aquaporin 2 antibody
    • Water channel protein for renal collecting duct antibody
    • WCH CD antibody
    • WCH-CD antibody
    • WCHCD antibody
    see all

Anti-Aquaporin 2 antibody 画像

  • ab62628 staining Aquaporin in Mouse embryonic kidney tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 1 hour at 20°C. An Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Immunofluorescence analysis of HeLa cells, using 1/500-1/1000 ab62628.

    Left image untreated.

    Right image treated with peptide.

Anti-Aquaporin 2 antibody (ab62628) 使用論文

This product has been referenced in:
  • Landegren N  et al. Autoantibodies Targeting a Collecting Duct-Specific Water Channel in Tubulointerstitial Nephritis. J Am Soc Nephrol 27:3220-3228 (2016). Read more (PubMed: 26984885) »
  • Steele SL  et al. Telomerase immortalization of principal cells from mouse collecting duct. Am J Physiol Renal Physiol 299:F1507-14 (2010). IF ; Mouse . Read more (PubMed: 20926633) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Sample Mouse Tissue sections (Embryonic kidney)
Specification Embryonic kidney
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
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投稿 Jun 25 2013

This antibody can react with both phosphorylated protein and non-phosphorylated protein at Ser256.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"