製品の概要

  • 製品名Anti-Aquaporin 2 antibody
    Aquaporin 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Aquaporin 2
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    A 15 aa C terminal peptide from rat AQP2.

  • ポジティブ・コントロール
    • Rat and human kidney cortex, apical membranes, inner and outer medulla (postranslational or glycosylation).
  • 特記事項In kidneys the AQP2 protein is mainly expressed in collecting ducts, which plays an important role in determining the final urinary output of water and solutes. The tissue section of this part will be a good positive control.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 29 kDa.
IHC-P Use a concentration of 2 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use a concentration of 0.5 - 1 µg/ml.

ターゲット情報

  • 機能Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • 組織特異性Expressed in renal collecting tubules.
  • 関連疾患Diabetes insipidus, nephrogenic, autosomal
  • 配列類似性Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • ドメインAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • 翻訳後修飾Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
  • 細胞内局在Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADH water channel antibody
    • AQP 2 antibody
    • AQP CD antibody
    • AQP-2 antibody
    • AQP-CD antibody
    • AQP2 antibody
    • AQP2_HUMAN antibody
    • AQPCD antibody
    • Aquaporin 2 collecting duct antibody
    • Aquaporin CD antibody
    • Aquaporin-2 antibody
    • Aquaporin-CD antibody
    • Aquaporin2 antibody
    • Aquaporine 2 antibody
    • Collecting duct water channel protein antibody
    • MGC34501 antibody
    • Water channel aquaporin 2 antibody
    • Water channel protein for renal collecting duct antibody
    • WCH CD antibody
    • WCH-CD antibody
    • WCHCD antibody
    see all

Anti-Aquaporin 2 antibody 画像

  • ab15081 staining Aquaporin 2 in rat kidney tissue section by IHC(P). Tissue underwent fixation in formalin, embedded in paraffin, heat mediated antigen retrieval in 10mM EDTA buffer pH 8.0 and blocking in 5% serum for 20 minutes at 20°C. The primary antibody, diluted 1/300 in TBSTween and incubated with sample for 1 hour at 20°C. A HRP conjugated goat polyclonal to rabbit was used undiluted as the secondary.  

    See Abreview

  • ab15081at a 1/250 dilution staining Aquaporin 2 in human kidney tissue by Immunohistochemistry (formalin fixed, paraffin embedded sections), incubated for 20 minutes at 25°C. Heat mediated retrieval step performed using EDTA at 100°C for 20 minutes. Secondary used undiluted monoclonal Goat anti-rabbit, Leica Refine, HRP polymer.

    See Abreview

Anti-Aquaporin 2 antibody (ab15081) 使用論文

This product has been referenced in:
  • Airik R  et al. Renal-Retinal Ciliopathy Gene Sdccag8 Regulates DNA Damage Response Signaling. J Am Soc Nephrol N/A:N/A (2014). Human . Read more (PubMed: 24722439) »
  • Hoppensack A  et al. A human in vitro model that mimics the renal proximal tubule. Tissue Eng Part C Methods 20:599-609 (2014). IHC-P ; Human . Read more (PubMed: 24266327) »

See all 6 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Kidney section - paraffin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization Yes - TritonX
Specification Kidney section - paraffin
Blocking step 1%BSA, 10% Goat serum in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Archana Raman

Verified customer

投稿 May 26 2016

Thank you for your email. Please find below the tetsing discount code for ab85379. At the bottom of the email, I have listed the information regarding the free of charge replacement for ab15081 and ab58968.
DISCOUNT CODE: xxx
Expiration date:...

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Thank you for your reply and the additional information. This is very helpful to us for our internal
I have checked our stock, but we have, unfortunately, only a new lot for ab15081 - which I'd be happy to send you free of charge. For ab7671 and a...

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Thank you for contacting us.
I am sorry to hear that all 3 antibodies are not performing as expected.
Before proceeding with the free of charge replacements, could you please let me know the following experimental details:
1) What was th...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Kidney)
Specification Kidney
Fixative 10% NBF
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS/EDTA pH 9.0
Permeabilization No
Blocking step Serum Free Protein Block as blocking agent for 5 minute(s) · Concentration: 0.25%
Username

Ms. Elizabeth Chlipala

Verified customer

投稿 Aug 20 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (kidney)
Specification kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, 100C, 20 minutes
Permeabilization No
Username

Abcam user community

Verified customer

投稿 Dec 02 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Kidney)
Specification Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 9,0
Permeabilization No
Blocking step Peroxidase Blocking solution (Dako) as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 May 06 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (kidney)
Specification kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM EDTA pH8.0
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Nov 06 2008

Thank you for getting back to me. The approach that you are employing is one that I would recommend and very similar to the approach that was used to test this antibody. We often find that some of our antibodies require a degree of optimisation as ...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I am in the process of contacting the lab with respect to the validation that would have been performed on the batch that you have been having...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"