The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa).
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
Belongs to the MIP/aquaporin (TC 1.A.8) family.
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
aquaporin 1 (Colton blood group) antibody
Aquaporin CHIP antibody
Channel forming integral protein 28kDa antibody
Channel like integral membrane protein 28 kDa antibody
CHIP 28 antibody
Colton blood group antibody
Growth factor induced delayed early response protein antibody
Urine water channel antibody
Water channel protein CHIP 29 antibody
Water channel protein CHIP29 antibody
Water channel protein for red blood cells and kidney proximal tubule antibody
Western blot - Anti-Aquaporin 1 antibody (ab87845)
All lanes : Anti-Aquaporin 1 antibody (ab87845) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431) Lane 2 : Human pancreas tissue lysate - total protein (ab29816) Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330) Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution