製品の概要

  • 製品名
    Anti-Aquaporin 1 antibody [7D11]
    Aquaporin 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [7D11] to Aquaporin 1
  • 特異性
    ab11025 recognises an epitope within an extracellular region of the water-specific channel aquaporin 1.
  • アプリケーション
    適用あり: IHC-P, IHC-Fr, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 198-208 of Rat Aquaporin 1.

  • ポジティブ・コントロール
    • Kidney tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    Purified from tissue culture supernatant.
  • ポリ/モノ
    モノクローナル
  • クローン名
    7D11
  • ミエローマ
    Sp2/0-Ag14
  • アイソタイプ
    IgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/500 - 1/1000.
WB 1/1000 - 1/2000. Predicted molecular weight: 32.28 kDa.
ELISA 1/1000 - 1/10000.

ターゲット情報

  • 機能
    Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • 組織特異性
    Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
  • 配列類似性
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • ドメイン
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AQP 1 antibody
    • AQP CHIP antibody
    • AQP-1 antibody
    • AQP1 antibody
    • AQP1_HUMAN antibody
    • aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
    • aquaporin 1 (Colton blood group) antibody
    • Aquaporin CHIP antibody
    • Aquaporin-1 antibody
    • Aquaporin-CHIP antibody
    • Aquaporin1 antibody
    • Channel forming integral protein 28kDa antibody
    • Channel like integral membrane protein 28 kDa antibody
    • CHIP 28 antibody
    • CHIP28 antibody
    • CO antibody
    • Colton blood group antibody
    • Growth factor induced delayed early response protein antibody
    • MGC26324 antibody
    • Urine water channel antibody
    • Water channel protein CHIP 29 antibody
    • Water channel protein CHIP29 antibody
    • Water channel protein for red blood cells and kidney proximal tubule antibody
    see all

参考文献

This product has been referenced in:
  • George SK  et al. Potential Use of Autologous Renal Cells from Diseased Kidneys for the Treatment of Renal Failure. PLoS One 11:e0164997 (2016). Read more (PubMed: 27776163) »
  • Kang HM  et al. Sox9-Positive Progenitor Cells Play a Key Role in Renal Tubule Epithelial Regeneration in Mice. Cell Rep 14:861-871 (2016). Read more (PubMed: 26776520) »

See all 4 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Specification
brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%
Username

Vlady Pavlova

Verified customer

投稿 Aug 16 2006

I'm sorry to hear you are experiencing problems with ab11025. This antibody has not been tested in immunofluorescence and this may be the reason for the problem. I would recommend trying a biotin-ABC-DAB detection system which is more sensitive that ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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