製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to APRT
  • アプリケーション
    適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide selected from the C-terminal region of Human APRT conjugated to KLH (NP_000476).

  • ポジティブ・コントロール
    • 293 cell lysates transiently transfected with the APRT gene.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab91428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 20 kDa.
ELISA 1/1000.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
  • パスウェイ
    Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.
  • 関連疾患
    Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD) [MIM:102600]; also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones.
  • 配列類似性
    Belongs to the purine/pyrimidine phosphoribosyltransferase family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adenine phosphoribosyltransferase antibody
    • AMP antibody
    • AMP diphosphorylase antibody
    • AMP pyrophosphorylase antibody
    • APRT antibody
    • APT_HUMAN antibody
    • DKFZp686D13177 antibody
    • MGC125856 antibody
    • MGC125857 antibody
    • MGC129961 antibody
    • Transphosphoribosidase antibody
    see all

画像

  • All lanes : Anti-APRT antibody (ab91428) at 1/100 dilution

    Lane 1 : 293 cell lysates (nontransfected)
    Lane 2 : 293 cell lysates transiently transfected with the APRT gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 20 kDa
  • ICC/IF image of ab91428 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91428, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab91428 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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