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Our Abpromise guarantee covers the use of ab16085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 1 µg/ml. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|ICC||Use a concentration of 5 µg/ml.|
ab16085 at 1ug/ml detecting membrane-bound human APRIL on HEK 293T cells by FACS analysis.
HEK 293T cells (5x105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of a non-cleavable mouse APRIL (thick line). Cells were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1ug/ml of MAb to APRIL (Sacha-1). After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.
Three days after transfection of cells with the indicated constructs, cells were fixed with acetone. Slides were blocked with IgG, and incubated for 1 hour with 5ug/ml ab16085 or control murine IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After washes wish PBS, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.
ab16085 has not yet been referenced specifically in any publications.