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Our Abpromise guarantee covers the use of ab109566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant Human APRIL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|Indirect ELISA||Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution). Allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human APRIL|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to APRIL (Biotin) (ab84029). ab84029 can be used as the detection antibody and ab109566 as the capture antibody. Detection limit for recombinant Human APRIL: 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.|
ab109566 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"