製品の概要

  • 製品名
    Anti-APPBP1 antibody - N-terminal
    APPBP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to APPBP1 - N-terminal
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide from the N-terminal region of human APPBP1 conjugated to KLH.

  • ポジティブ・コントロール
    • APPBP1-transfected 293 cells lysate; human brain tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/50 - 1/100. Predicted molecular weight: 60 kDa.
IHC-P 1/10 - 1/50.

ターゲット情報

  • 機能
    Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
  • 組織特異性
    Ubiquitous in fetal tissues. Expressed throughout the adult brain.
  • パスウェイ
    Protein modification; protein neddylation.
  • 配列類似性
    Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.
  • 細胞内局在
    Cell membrane. Colocalizes with APP in lipid rafts.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 59 kDa antibody
    • A 116A10.1 antibody
    • Amyloid beta precursor protein binding protein 1 59kD antibody
    • Amyloid beta precursor protein binding protein 1 59kDa antibody
    • Amyloid beta precursor protein binding protein 1 antibody
    • Amyloid beta precursor protein binding protein 1, 59 kDa antibody
    • Amyloid beta precursor protein-binding protein 1 antibody
    • Amyloid protein binding protein 1 antibody
    • Amyloid protein-binding protein 1 antibody
    • APP BP1 antibody
    • APP-BP1 antibody
    • APPBP 1 antibody
    • APPBP1 antibody
    • HPP 1 antibody
    • HPP1 antibody
    • NAE 1 antibody
    • NAE1 antibody
    • NEDD8 activating enzyme E1 regulatory subunit antibody
    • NEDD8 activating enzyme E1 subunit 1 antibody
    • NEDD8 activating enzyme E1 subunit antibody
    • NEDD8-activating enzyme E1 regulatory subunit antibody
    • Proto oncogene protein 1 antibody
    • Proto-oncogene protein 1 antibody
    • Protooncogene protein 1 antibody
    • ula 1 antibody
    • ULA1_HUMAN antibody
    see all

画像

  • All lanes : Anti-APPBP1 antibody - N-terminal (ab71542) at 1/50 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the APPBP1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 60 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Analysis of APPBP1 expression in formalin-fixed, paraffin embedded human brain tissue using ab71542, at 1/10 dilution (peroxidase-conjugated secondary antibody; DAB staining).

参考文献

ab71542 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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