製品の概要

  • 製品名ApoTrack™ Cytochrome c Apoptosis ICC Antibody Kit
    ApoTrack™ Cytochrome c Apoptosis キット 製品一覧
  • 検出方法Fluorescent
  • サンプルの種類
    Adherent cells
  • アッセイタイプDirect
  • 種交差性
    交差種: Mouse, Rat, Human
  • 製品の概要

    Apoptotic cells which have released mitochondrial cytochrome c into the cytosol can be differentiated from non apoptotic cells which still retain cytochrome c in their mitochondria by fluorescence microscopy.

    ab110417 (MSA07) contains a cytochrome c monoclonal antibody (50 µg) and an isotype-specific secondary antibody (50 µg) which has a fluorescein fluorophore conjugated to it. The kit also contains an ATP synthase V subunit alpha monoclonal antibody (50 µg) and the appropriate isotype-specific secondary antibody (50 µg) which has a Texas Red fluorophore conjugated to it.

    ATP synthase is an inner mitochondrial membrane protein and serves as a mitochondrial marker since it is not released into the cytoplasm during apoptosis, unlike cytochrome c. Thus one is able to observe cytochrome c and a mitochondrial marker (ATP synthase subunit alpha) simultaneously by fluorescence microscopy using ab110417. Buffers and blocking solution for immunocytochemical analysis are also included in the kit.

  • 特記事項

    Store Goat Serum at -20°C. Store all other components at 4°C.

  • アプリケーション適用あり: ICC/IFmore details
  • 試験プラットフォームReagents

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab110417 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    ICC/IF Use at an assay dependent concentration.

    ApoTrack™ Cytochrome c Apoptosis ICC Antibody Kit 画像

    プロトコール

    ApoTrack™ Cytochrome c Apoptosis ICC Antibody Kit (ab110417) 使用論文

    This product has been referenced in:
    • Ismail AM  et al. Extra virgin olive oil potentiates the effects of aromatase inhibitors via glutathione depletion in estrogen receptor-positive human breast cancer (MCF-7) cells. Food Chem Toxicol 62:817-24 (2013). Human . Read more (PubMed: 24161488) »
    • Napolitano M  et al. 17ß-Estradiol protects cerebellar granule cells against ß-amyloid-induced toxicity via the apoptotic mitochondrial pathway. Neurosci Lett N/A:N/A (2013). Read more (PubMed: 24287378) »

    See all 6 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"