製品の概要

  • 製品名Apoptotic DNA Ladder Isolation Kit
    DNA fragments キット 製品一覧
  • テスト
    50 x 1 assay
  • サンプルの種類
    Tissue, Adherent cells, Suspension cells
  • アッセイタイプDirect
  • 製品の概要

    Apoptotic DNA Ladder Isolation Kit (ab65627) provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells and tissues. The new procedure selectively extracts DNA ladders without interference of intact genomic DNA in only 2-4 hours. As little as 5% of apoptotic cells in a sample can be visualized with this high sensitivity assay.



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  • 特記事項

    Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.

アプリケーション

Our Abpromise guarantee covers the use of ab65627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Apoptotic DNA Ladder Isolation Kit 画像

  • Apoptosis induced in Jurkat cells (1e6 cell x mL-1) by incubation for 24 hours with 1) Control medium; 2) 2 uM Camptothecin (ab120115); 3) 10 uM Camptothecin; 4) 10 uM Camptothecin + 20 nM caspase inhibitor Z-VAD(OMe)-FMK (zVAD; ab120487); 5) 50 ng x mL-1 anti-Fas Ab (MBL), 6) 0.05 mM H2O2 (Sigma); 7) 1 mM H2O2. 5 uL of DNA preparation loaded into each well.

  • DNA ladders were extracted from 2 x 106 Jurkat cells were treated with 2 μM Camptothecin. The kit extracts laddered DNA only but not intact genomic DNA; therefore, more cells can be extracted and load on the agarose gel. Lane 1: 1 kb DNA ladder; Lane 2: 6 hour induction; Lane 3: 12 hour induction.

プロトコール

Apoptotic DNA Ladder Isolation Kit (ab65627) 使用論文

This product has been referenced in:
  • Mochizuki S  et al. Effect of ADAM28 on carcinoma cell metastasis by cleavage of von Willebrand factor. J Natl Cancer Inst 104:906-22 (2012). Mouse . Read more (PubMed: 22636800) »

See 1 Publication for this product

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The DNA suspension buffer already contains glycerol to help keep the sample in the well. The electric current should not be started immediately after adding the samp...

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