Apoptotic DNA Ladder Detection Kit (ab66090)

製品の概要

  • 製品名Apoptotic DNA Ladder Detection Kit
    DNA fragments キット 製品一覧
  • テスト
    50 x 1 assay
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプDirect
  • 全工程の試験時間
    1h 30m
  • 製品の概要

    Apoptotic DNA Ladder Detection Kit (ab66090) provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells. It can isolate small fragmented DNA from cells in only 90 minutes, whereas other similar products in the market require 1-2 days. No DNA extraction or additional columns required.



    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.

アプリケーション

Our Abpromise guarantee covers the use of ab66090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

Apoptotic DNA Ladder Detection Kit 画像

  • Apoptosis was induced in 10e6 Jurkat cells by treatment with 10 μM Camptothecin (CPT) (ab120115) for 4 hours (2, 5) or 2 μM CPT for 24 hours (3, 6) whilst control cells were kept without treatment (1, 4). DNA was extracted and resuspended in 60 uL resuspension buffer. 10 uL (1-3) or 40 uL (4-6) were loaded onto the agarose gel.

プロトコール

Apoptotic DNA Ladder Detection Kit (ab66090) 使用論文

ab66090 has not yet been referenced specifically in any publications.

Product Wall

If you choose to do an overnight incubation with Enzyme B, we recommend doing so at 50 degrees Celsius. If you do this, make sure the tube is sealed well to prevent evaporation.



As long as you perform the incubation for at least 30 m...

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It is very easy to use.

Excellent Excellent 5/5 (Ease of Use)
Abreviews
very good for menuscript data
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Miss. Jae Ok SEO

Verified customer

投稿 Jul 23 2014

While it is uncommon for the DNA pellet to not break up with vortexing and pippetting, should this occur warming the solutions should help. There is also a yellow dye included in the suspension buffer for visualizing on the gel.

The lab has confirmed that you may store the resuspended DNA at -20°C. DNA generally does not freeze at this temp. Please don’t store it at 4°C.

Thank you for your enquiry.

For India, you can order from one of our following official distributors. I can recommend to contact them and they can give you a price.

Central and South India:

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Thank you for contacting Abcam.

I have had the chance to speak with the lab. Although they have not seen this type of issue before, they suggest when doing overnight incubation to ensure that the tubes are airtight to prevent evaporation of ...

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Thank you for your enquiry. I am pleased to confirm the following information for you regarding the components of this kit (ab66090) TE Lysis Buffer- Aids in cell lysis Enzyme A Solution- Removes RNA Enzyme B (Lyophilized)- Digests ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"