Anti-Apoptosis inhibitor 5 抗体 (ab99307)

製品の概要

  • 製品名Anti-Apoptosis inhibitor 5 antibody
    Apoptosis inhibitor 5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Apoptosis inhibitor 5
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Immunogen corresponds to a region within amino acids 1-50 of Human Apoptosis inhibitor 5 (XP_508375.2).

  • ポジティブ・コントロール
    • Whole cell lysates from HeLa, 293T and Mouse NIH 3T3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab99307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
  • 組織特異性Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer.
  • 配列類似性Belongs to the API5 family.
  • ドメインTwo regions, an N-terminal (aa 96-107) and a C-terminal (aa 274-311) are required for binding FGF2.
  • 細胞内局在Cytoplasm and Nucleus. Cytoplasm. Mainly nuclear. Can also be cytoplasmic.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AAC 11 antibody
    • AAC-11 antibody
    • AAC11 antibody
    • Antiapoptosis clone 11 protein antibody
    • API 5 antibody
    • API-5 antibody
    • api5 antibody
    • API5 like 1 antibody
    • API5_HUMAN antibody
    • API5L1 antibody
    • Apoptosis inhibitor 5 antibody
    • Cell migration inducing gene 8 protein antibody
    • Cell migration-inducing gene 8 protein antibody
    • Fibroblast growth factor 2 interacting factor 2 antibody
    • Fibroblast growth factor 2-interacting factor antibody
    • FIF antibody
    • MIG 8 antibody
    • MIG8 antibody
    • Migration inducing protein MIG8 antibody
    • Protein XAGL antibody
    see all

Anti-Apoptosis inhibitor 5 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling Apoptosis inhibitor 5 with ab99307 at 1/1000 (0.2µg/mg) and 1/200 (1 µg/mg). Detection: DAB.
  • All lanes : Anti-Apoptosis inhibitor 5 antibody (ab99307) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 58 kDa


    Exposure time : 10 seconds
  • ab99307, at 0.4 µg/ml, staining Apoptosis inhibitor 5 in HeLa whole cell lysate immunoprecipitated using ab99307 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 1 second.

Anti-Apoptosis inhibitor 5 antibody (ab99307) 使用論文

ab99307 has not yet been referenced specifically in any publications.

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