Anti-Apoptosis enhancing nuclease antibody
Rabbit polyclonal to Apoptosis enhancing nuclease
Synthetic peptide, corresponding to a region within amino acids 236-297 of Apoptosis enhancing nuclease (NP_073604).
HeLa, HepG2 or MOLT4 cell lines.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 36 kDa.
1/100 - 1/250.
Exonuclease with activity against single- and double-stranded DNA and RNA. Mediates p53-induced apoptosis. When induced by p53 following DNA damage, digests double-stranded DNA to form single-stranded DNA and amplifies DNA damage signals, leading to enhancement of apoptosis.
Contains 1 exonuclease domain.
Nucleus. Nucleus > nucleolus. Localized predomintly in the nucleolus. Translocates from the nucleolus to the nucleoplasm upon apoptosis induction.
Information by UniProt
apoptosis enhancing nuclease antibody
Western blot - Apoptosis enhancing nuclease antibody (ab97915)
Anti-Apoptosis enhancing nuclease antibody (ab97915) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 36 kDa 10% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Apoptosis enhancing nuclease antibody (ab97915)
ab97915, at 1/100 dilution, staining Apoptosis enhancing nuclease in Cal27 xenograft tissue by Immunohistochemistry. Formalin-fixed, paraffin-embedded section.
has not yet been referenced specifically in any publications.
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