The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml.
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa).
機能Exonuclease with activity against single- and double-stranded DNA and RNA. Mediates p53-induced apoptosis. When induced by p53 following DNA damage, digests double-stranded DNA to form single-stranded DNA and amplifies DNA damage signals, leading to enhancement of apoptosis.
配列類似性Contains 1 exonuclease domain.
細胞内局在Nucleus. Nucleus > nucleolus. Localized predomintly in the nucleolus. Translocates from the nucleolus to the nucleoplasm upon apoptosis induction.