Anti-Apolipoprotein M 抗体 (ab76458)

製品の概要

  • 製品名Anti-Apolipoprotein M antibody
    Apolipoprotein M 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Apolipoprotein M
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Pig, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Apolipoprotein M.

    (Peptide available as ab87119.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Plasma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 21 kDa).

ターゲット情報

  • 機能Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
  • 組織特異性Plasma protein. Expressed in liver and kidney.
  • 配列類似性Belongs to the calycin superfamily. Lipocalin family. Highly divergent.
  • 細胞内局在Secreted. Present in high density lipoprotein (HDL) and to a lesser extent in triglyceride-rich lipoproteins (TGRLP) and low density lipoproteins.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apo M antibody
    • Apo-M antibody
    • Apolipoprotein M antibody
    • ApoM antibody
    • APOM_HUMAN antibody
    • G3A antibody
    • HSPC336 antibody
    • MGC22400 antibody
    • NG20 antibody
    • NG20 like protein antibody
    • Protein G3a antibody
    see all

Anti-Apolipoprotein M antibody 画像

  • Anti-Apolipoprotein M antibody (ab76458) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab76458 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76458, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 cells at 5µg/ml.
  • IHC image of Apolipoprotein M staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76458, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Apolipoprotein M antibody (ab76458) 使用論文

ab76458 has not yet been referenced specifically in any publications.

Product Wall

Merci de nous avoir contactés.

J’ai vérifié ab76458. Puisque ce produit a une concentration >1mg/ml de BSA, je peux vous confirmer qu’il ne contient pas de BSA. Notez bien que ceci n’est pas le cas p...

Read More

Merci de nous avoir contactés.

ab91656 n’a pas été purifié. Il est vendu sous forme de surnageant et dans un tampon de 49% PBS, 0.01% Azoture de sodium, 50% Glycérol, 0.05% BSA.

ab76458 a &ea...

Read More

Merci de nous avoir contactés.

Comme je vous l’ai expliqué au téléphone, la séquence immunogène d’ab91656 est xxxxxxxx qui correspond aux acides aminés xxxxxxxchez l’humain. J...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"