Anti-Apolipoprotein L 1 抗体 [EPR2907(2)] (ab108315)


  • 製品名Anti-Apolipoprotein L 1 antibody [EPR2907(2)]
    Apolipoprotein L 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2907(2)] to Apolipoprotein L 1
  • アプリケーション適用あり: WB, IP, ICC, Flow Cytmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Apolipoprotein L 1.

  • ポジティブ・コントロール
    • Human plasma, ovary cancer, and HepG2 cell lysates.
  • 特記事項


    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab108315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IP 1/10 - 1/100.
ICC 1/100 - 1/250.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.
    • 組織特異性Plasma. Found on APOA-I-containing high density lipoprotein (HDL3). Expressed in pancreas, lung, prostate, liver, placenta and spleen.
    • 関連疾患Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4) [MIM:612551]. It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
    • 配列類似性Belongs to the apolipoprotein L family.
    • 翻訳後修飾Phosphorylation sites are present in the extracelllular medium.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • APO L antibody
      • Apo-L antibody
      • ApoL antibody
      • APOL I antibody
      • ApoL-I antibody
      • APOL1 antibody
      • APOL1_HUMAN antibody
      • APOLI antibody
      • Apolipoprotein L antibody
      • Apolipoprotein L I antibody
      • Apolipoprotein L-I antibody
      • Apolipoprotein L1 antibody
      • FSGS4 antibody
      see all

    Anti-Apolipoprotein L 1 antibody [EPR2907(2)] 画像

    • All lanes : Anti-Apolipoprotein L 1 antibody [EPR2907(2)] (ab108315) at 1/1000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : Human ovary cancer lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 44 kDa
    • Apolipoprotein L 1  was immunoprecipitated from 1mg of Human plasma with ab108315 at 1/40 dilution.

      Western blot was performed from the immunoprecipitate using ab108315 at 1/1000 dilution.

      Goat Anti-Rabbit IgG peroxidase conjugated,was used as secondary antibody at 1/1000 dilution.

      Lane 1:Human plasma

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds.

    • Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2(Human hepatocellular carcinoma) cell line labeling Apolipoprotein L 1 with ab108315 at 1/70 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Green). Goat anti Rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

    Anti-Apolipoprotein L 1 antibody [EPR2907(2)] (ab108315) 使用論文

    This product has been referenced in:
    • Ma L  et al. Localization of APOL1 Protein and mRNA in the Human Kidney: Nondiseased Tissue, Primary Cells, and Immortalized Cell Lines. J Am Soc Nephrol N/A:N/A (2014). Read more (PubMed: 25012173) »
    • Beyer M  et al. High-resolution transcriptome of human macrophages. PLoS One 7:e45466 (2012). WB ; Human . Read more (PubMed: 23029029) »

    See all 2 Publications for this product

    Product Wall

    The lab uses Gibco FreeStyle 293 Expression medium, which is serum and protein free.

    For ab126615, I have attached the WB image with HepG2 cell lysate.

    As for the cell lysis buffer used for the WB with ab108315, it is product ab152163 ( that was used. The buffer composition is g...

    Read More