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Our Abpromise guarantee covers the use of ab1906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 21163940|
|IP||Use at an assay dependent concentration. PubMed: 21163940|
|WB||1/1000 - 1/10000. Predicted molecular weight: 38 kDa.|
|IHC-P||1/1000 - 1/100000. Antigen retrieval is not essential but may optimise staining.
The staining intensity of formalin-fixed paraffin embedded tissues may be significantly improved by pretreatment methods such as: 70% Formic acid for 10-30 minutes at room temperature or Hydrolytic autoclaving.
|IHC-Fr||1/1000 - 1/100000.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 22896615
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
This image is courtesy of an anonymous Abreview
Blocked with 5% milk for 1 hour at 22°C.
Incubated with the primary antibody in 5% milk for 16 hours at 4°C.
ab1906 staining Apolipoprotein E in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/200 in TBS/BSA/azide) for 2 hours at 21°C. An undiluted biotin-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
ab1906 staining Apolipoprotein E in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab1906 at 1/100 in blocking solution for 16h at 4°C. An Alexa Fluor® 647 conjugated goat anti-mouset polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. Apolipoprotein E expression can be observed in Muller cells.
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