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Full length native protein (purified)(Human) (isolated from human plasma by density gradient centrifugation followed by HPLC purification).
Our Abpromise guarantee covers the use of ab7620 in the following tested applications.
|Indirect ELISA||1/4000 - 1/8000.|
|WB||1/5000 - 1/10000. Predicted molecular weight: 36 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
This image is courtesy of an Abreview submitted by Fabien GosseletBlocking Step: 3% Milk for 20 minutes at room temperature
ICC/IF image of ab7620 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab7620 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.