Anti-Apolipoprotein D 抗体 [EPR2916] (ab108191)

製品の概要

  • 製品名Anti-Apolipoprotein D antibody [EPR2916]
    Apolipoprotein D 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2916] to Apolipoprotein D
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Apolipoprotein D.

  • ポジティブ・コントロール
    • WB: Human plasma cell lysate IHC-P: Human kidney or liver tissue
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ターゲット情報

  • 機能APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.
  • 組織特異性Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears.
  • 配列類似性Belongs to the calycin superfamily. Lipocalin family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APO D antibody
    • Apo-D antibody
    • ApoD antibody
    • APOD protein antibody
    • APOD_HUMAN antibody
    • Apolipoprotein D antibody
    • ApolipoproteinD antibody
    see all

Anti-Apolipoprotein D antibody [EPR2916] 画像

  • Anti-Apolipoprotein D antibody [EPR2916] (ab108191) at 1/2000 dilution (purified) + human plasma at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 21 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human liver with purified ab108191 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Anti-Apolipoprotein D antibody [EPR2916] (ab108191) at 1/1000 dilution (unpurified) + Human plasma cell lysate at 10 µg

    Predicted band size : 21 kDa
  • Immunohistochemical staining of Apolipoprotein D in paraffin embedded human kidney tissue, using unpurified ab108191 at a 1/100 dilution.

  • Immunohistochemical staining of Apolipoprotein D in paraffin embedded Human liver tissue, using unpurified ab108191 at a 1/100 dilution.

Anti-Apolipoprotein D antibody [EPR2916] (ab108191) 使用論文

ab108191 has not yet been referenced specifically in any publications.

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