Anti-Apolipoprotein CIII 抗体 [EP1372Y] (ab76305)

製品の概要

  • 製品名
    Anti-Apolipoprotein CIII antibody [EP1372Y]
    Apolipoprotein CIII 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1372Y] to Apolipoprotein CIII
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to the C terminus of human Apolipoprotein CIII.

  • ポジティブ・コントロール
    • Human liver tissue lysate, Rat primary astrocytes.
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IP 1/30.

ターゲット情報

  • 機能
    Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
  • 組織特異性
    Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
  • 関連疾患
    Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
  • 配列類似性
    Belongs to the apolipoprotein C3 family.
  • 翻訳後修飾
    O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APOC3 antibody
    • APO C3 antibody
    • Apo CIII antibody
    • Apo-CIII antibody
    • APOC 3 antibody
    • ApoC III antibody
    • ApoC-III antibody
    • APOC3 antibody
    • APOC3_HUMAN antibody
    • ApoCIII antibody
    • Apolipoprotein C III antibody
    • Apolipoprotein C-III antibody
    • Apolipoprotein C3 antibody
    • ApolipoproteinCIII antibody
    • MGC150353 antibody
    see all

画像

  • Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305) at 1/2000 dilution + Human liver tissue lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11 kDa

参考文献

This product has been referenced in:
  • Wang Y  et al. Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman primate serum. J Lipid Res 52:1265-71 (2011). Read more (PubMed: 21371998) »

See 1 Publication for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary astrocytes culture)
Permeabilization
Yes - 0.1M PBS with 1% Triton X
Specification
Primary astrocytes culture
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Methanol
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Sep 06 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Liver)
Permeabilization
Yes - 0.1 M PBS with 3% Triton X
Specification
Liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Feb 05 2016

Thank you for contacting us.

Please find attached the WB protocol;

http://www.abcam.com/index.html?pageconfig=resource&rid=13045

Please find attached the link which actually explains the reason of band at ˜70-80 kDa. T...

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (BREAST (MCF-7))
Loading amount
0.2 µg
Specification
BREAST (MCF-7)
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Jul 01 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (U266 cell line B lymphocyte (myeloma))
Permeabilization
No
Gating Strategy
total stained cells
Specification
U266 cell line B lymphocyte (myeloma)
Preparation
Cell harvesting/tissue preparation method: suspension cells, so used directly
Sample buffer: RPMI 1640 medium with 2 mM L-glutamine
Fixation
Unfixed
Username

Prof. Paul Townsend

Verified customer

投稿 May 07 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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