Anti-Apolipoprotein CIII 抗体 [EP1372Y] (ab76305)

製品の概要

  • 製品名
    Anti-Apolipoprotein CIII antibody [EP1372Y]
    Apolipoprotein CIII 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1372Y] to Apolipoprotein CIII
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to the C terminus of human Apolipoprotein CIII.

  • ポジティブ・コントロール
    • Human liver tissue lysate, Rat primary astrocytes.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IP 1/30.

ターゲット情報

  • 機能
    Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
  • 組織特異性
    Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
  • 関連疾患
    Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
  • 配列類似性
    Belongs to the apolipoprotein C3 family.
  • 翻訳後修飾
    O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APOC3 antibody
    • APO C3 antibody
    • Apo CIII antibody
    • Apo-CIII antibody
    • APOC 3 antibody
    • ApoC III antibody
    • ApoC-III antibody
    • APOC3 antibody
    • APOC3_HUMAN antibody
    • ApoCIII antibody
    • Apolipoprotein C III antibody
    • Apolipoprotein C-III antibody
    • Apolipoprotein C3 antibody
    • ApolipoproteinCIII antibody
    • MGC150353 antibody
    see all

Anti-Apolipoprotein CIII antibody [EP1372Y] 画像

  • Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305) at 1/2000 dilution + Human liver tissue lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11 kDa

Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305) 使用論文

ab76305 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary astrocytes culture)
Permeabilization
Yes - 0.1M PBS with 1% Triton X
Specification
Primary astrocytes culture
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Methanol
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Sep 06 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Liver)
Permeabilization
Yes - 0.1 M PBS with 3% Triton X
Specification
Liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Feb 05 2016

Thank you for contacting us.

Please find attached the WB protocol;

http://www.abcam.com/index.html?pageconfig=resource&rid=13045

Please find attached the link which actually explains the reason of band at ˜70-80 kDa. T...

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (BREAST (MCF-7))
Loading amount
0.2 µg
Specification
BREAST (MCF-7)
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Jul 01 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (U266 cell line B lymphocyte (myeloma))
Permeabilization
No
Gating Strategy
total stained cells
Specification
U266 cell line B lymphocyte (myeloma)
Preparation
Cell harvesting/tissue preparation method: suspension cells, so used directly
Sample buffer: RPMI 1640 medium with 2 mM L-glutamine
Fixation
Unfixed
Username

Prof. Paul Townsend

Verified customer

投稿 May 07 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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