Sandwich ELISA - Anti-Apolipoprotein CIII antibody (Biotin) (ab21024)Image from Wang Y et al., J Lipid Res. 2011 Jun;52(6):1265-71. Epub 2011 Mar 2. Fig 2.; doi: 10.1194/jlr.D011148; June 2011, The Journal of Lipid Research, 52, 1265-1271.
Sandwich ELISA, detecting Apolipoprotein CIII levels in Human or cynomolgus monkey serum, using ab21024 as detection antibody.
Anti-human Apolipoprotein CIII polyclonal antibody (ab21032) was added to an ELISA plate and incubated overnight at 4°C. Blocking buffer was added and incubated for 1 hour at room temperature. 50 µl of serum or standard was added to each well and incubated for 2 hours at room temperature. After washing, 50 µl/well of detection antibody (ab21024 at 1 µg/ml) was added and incubated at room temperature 1 hour followed by 50 µl/well of Streptavidin-HRP (ab64269). After color development, the absorbance was read at 450nm.
Wang Y et al. Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman primate serum. J Lipid Res52:1265-71 (2011).
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Dernick G et al. Multidimensional profiling of plasma lipoproteins by size exclusion chromatography followed by reverse-phase protein arrays. J Lipid Res52:2323-31 (2011).
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