製品の概要

  • 製品名Anti-Apolipoprotein CIII antibody
    Apolipoprotein CIII 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Apolipoprotein CIII
  • 特異性Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. This antibody reacts with human apoLipoprotein C-III and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, E and J apoLipoproteins.
  • アプリケーション適用あり: ELISA, IHC-P, WB, IPmore details
  • 種交差性
    交差種: Human, Syrian Hamster
  • 免疫原

    Full length native apoLipoprotein Type C-III (purified).

  • 特記事項This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/5000 - 1/10000.
IHC-P 1/50 - 1/200.
WB 1/500 - 1/5000.
IP 1/100.

ターゲット情報

  • 機能Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
  • 組織特異性Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
  • 関連疾患Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
  • 配列類似性Belongs to the apolipoprotein C3 family.
  • 翻訳後修飾O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APOC3 antibody
    • APO C3 antibody
    • Apo CIII antibody
    • Apo-CIII antibody
    • APOC 3 antibody
    • ApoC III antibody
    • ApoC-III antibody
    • APOC3 antibody
    • APOC3_HUMAN antibody
    • ApoCIII antibody
    • Apolipoprotein C III antibody
    • Apolipoprotein C-III antibody
    • Apolipoprotein C3 antibody
    • ApolipoproteinCIII antibody
    • MGC150353 antibody
    see all

Anti-Apolipoprotein CIII antibody 画像

  • IHC image of ab7619 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7619, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Apolipoprotein CIII antibody (ab7619) 使用論文

This product has been referenced in:
  • Hesse D  et al. Hepatic trans-Golgi action coordinated by the GTPase ARFRP1 is crucial for lipoprotein lipidation and assembly. J Lipid Res 55:41-52 (2014). IHC-P ; Mouse . Read more (PubMed: 24186947) »
  • Qu S  et al. PPAR{alpha} mediates the hypolipidemic action of fibrates by antagonizing FoxO1. Am J Physiol Endocrinol Metab 292:E421-34 (2007). WB ; Syrian Hamster . Read more (PubMed: 16985262) »

See all 4 Publications for this product

Product Wall

Thank you again for your enquiry and I apologize for the delay in responding to you. There is no Western blot image available from us for this antibody. This antibody has been used and the Western images published in the following reference, however,...

Read More

We apply purified apolipoprotein C-III to the gel and get only a single band at 8.8 kDa. A picture is not available. tThe suggested dilution is 1/5000 to 1/10,000. Human serum would be a good western sample. I hope this information helps you. If yo...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"