Anti-Apolipoprotein B 抗体 (ab7616)
Key features and details
- Goat polyclonal to Apolipoprotein B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Apolipoprotein B antibody
Apolipoprotein B 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Apolipoprotein B -
由来種
Goat -
特異性
Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Full length native apoLipoprotein Type B (purified).
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特記事項
This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.
At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Abcam's apo-lipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 4.7625% Sodium borate, 0.146% EDTA, 0.435% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. -
一次抗体 備考
At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Abcam's apo-lipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7616の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/200 - 1/1000.
|
特記事項 |
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WB
1/200 - 1/1000. |
ターゲット情報
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機能
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. -
関連疾患
Hypobetalipoproteinemia, familial, 1
Familial ligand-defective apolipoprotein B-100
Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. -
配列類似性
Contains 1 vitellogenin domain. -
翻訳後修飾
Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. -
細胞内局在
Cytoplasm. Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 338 Human
- Omim: 107730 Human
- SwissProt: P04114 Human
- Unigene: 120759 Human
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別名
- Apo B 100 antibody
- Apo B antibody
- Apo B-100 antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab7616 は 18 報の論文で使用されています。
- Yang W et al. Role of diacylglycerol O-acyltransferase (DGAT) isoforms in bovine hepatic fatty acid metabolism. J Dairy Sci 105:3588-3600 (2022). PubMed: 35181144
- Yang W et al. Role of sortilin 1 (SORT1) on lipid metabolism in bovine liver. J Dairy Sci 105:5420-5434 (2022). PubMed: 35469640
- Takahashi Y et al. Organoid-derived intestinal epithelial cells are a suitable model for preclinical toxicology and pharmacokinetic studies. iScience 25:104542 (2022). PubMed: 35754737
- Wang B et al. Hepatitis C virus induces oxidation and degradation of apolipoprotein B to enhance lipid accumulation and promote viral production. PLoS Pathog 17:e1009889 (2021). PubMed: 34492079
- Tougan T et al. The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity. Malar J 19:155 (2020). PubMed: 32295584