製品の概要

  • 製品名Anti-Apolipoprotein B antibody
    Apolipoprotein B 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Apolipoprotein B
  • 特異性Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins.
  • アプリケーション適用あり: Dot blot, ELISA, IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Rabbit, Cow, Human
  • 免疫原

    Full length native apoLipoprotein Type B (purified).

  • ポジティブ・コントロール
    • ICC/IF: HepG2 cells.
  • 特記事項

    This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.

    At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Abcam's apo-lipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot 1/5000 - 1/10000.
ELISA 1/2000 - 1/10000.
IHC-P 1/50 - 1/200.
WB 1/200 - 1/1000.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • 関連疾患Hypobetalipoproteinemia, familial, 1
    Familial ligand-defective apolipoprotein B-100
    Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
  • 配列類似性Contains 1 vitellogenin domain.
  • 翻訳後修飾Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
  • 細胞内局在Cytoplasm. Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apo B-100 antibody
    • Apo B-48 antibody
    • ApoB 100 antibody
    • ApoB 48 antibody
    • APOB antibody
    • APOB_HUMAN antibody
    • Apolipoprotein B 100 antibody
    • Apolipoprotein B antibody
    • Apolipoprotein B-48 antibody
    • Apolipoprotein B48 antibody
    • FLDB antibody
    • LDLCQ4 antibody
    see all

Anti-Apolipoprotein B antibody 画像

  • ab7616 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab7616 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Apolipoprotein B antibody (ab7616) 使用論文

This product has been referenced in:
  • Shimizu M  et al. Quercetin represses apolipoprotein B expression by inhibiting the transcriptional activity of C/EBPß. PLoS One 10:e0121784 (2015). WB ; Human . Read more (PubMed: 25875015) »
  • Liu L  et al. Effects of nonesterified fatty acids on the synthesis and assembly of very low density lipoprotein in bovine hepatocytes in vitro. J Dairy Sci 97:1328-35 (2014). Cow . Read more (PubMed: 24377799) »

See all 8 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization No
Specification Liver
Blocking step Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde
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投稿 Nov 12 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization No
Specification Liver
Blocking step Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 10 2015

Thank you for contacting us.

The gel that was used was Tris-Acetate. We use Invitrogen's NuPAGE 3-8% TA gels: http://products.invitrogen.com/ivgn/product/EA03755BOX
I hope this information is helpful to you. Please do not hesitate to co...

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Thank you for contacting us.
I have contacted the lab asking for the protocol used to produce the WB shown on the datasheet. Here is the answer I got:
Ab77859 was used at 0.01 ug as shown in the WB. When we used the protein at higher concentr...

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Vielen Dank für Ihren Anruf.
Tatsächlich hat eine meiner Kolleginnen mit der Turbidimetrie gearbeitet, und nach einiger Diskussion sind wir der Meinung, dass für Ihren Kunden Antikörper, die in ELISA und/oder IP funktionieren, geeignet sein sollte...

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Thank you for contacting us.


We have not mapped the epitope for ab7616 and have not tested the antibody against Apo B48 and B100. We have no information about the cross reactivity to Apo B48 and B100.

I am sorry for not being...

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Thank you for getting back to us with further details. I am handling Charlottes enquiries in her absence. Once again I am sorry to hear that you have been having difficulties with this antibody. The problems that you have been having with this anti...

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Thank you for your enquiry. This is a polyclonal antibody and will therefore consist of a mixed population of IgGs each of which will recognise a different epitope. I am not sure that the antibody can be used to prevent the Apo B100 antibody from...

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Thank you for your enquiry. When ab7616 was tested for application in Western blotting, it was tested against purified Apo B protein, which would be recommended as a positive control. If you have any additional questions, please contact us again.

Thank you for your email. Ab7616 was made against the whole apo B and should be able to recognize all the residues. I'm not familiar with the antibody from Polymedco, so unfortunately can't state how this antibody works in comparison. If you have any m...

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