This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Apolipoprotein B 抗体 (ab50069)

製品の概要

関連製品

アプリケーション

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

アプリケーション Abreviews 特記事項
IP
  • 追加情報IHC-P: see figure below
    This antibody gave a positive result in ELISA against the immunizing peptide (ab50068).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
    • 関連疾患Hypobetalipoproteinemia, familial, 1
      Familial ligand-defective apolipoprotein B-100
      Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
    • 配列類似性Contains 1 vitellogenin domain.
    • 翻訳後修飾Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
    • 細胞内局在Cytoplasm. Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Apo B-100 antibody
      • Apo B-48 antibody
      • ApoB 100 antibody
      • ApoB 48 antibody
      • APOB antibody
      • APOB_HUMAN antibody
      • Apolipoprotein B 100 antibody
      • Apolipoprotein B antibody
      • Apolipoprotein B-48 antibody
      • Apolipoprotein B48 antibody
      • FLDB antibody
      • LDLCQ4 antibody
      see all

    Anti-Apolipoprotein B antibody 画像

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • IHC image of Apoliporotein B staining in human liver carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50069, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    • Immunoprecipitation of Apolipoprotein B, using a Huh7.5 human hepatoma cell line conditioned medium. The protein was immunoprecipitated from 1.8 mL spent medium, using 29µg of Rabbit polyclonal to Apolipoprotein B (ab50069). A different Apolipoprotein B antibody was used for the western blot.

       

      Lanes 1: Pull down with ab50069, Lane 2: IP control with rabbit IgG.

       

      Band: >200kDa: Apolipoprotein B.

      See Abreview

    Anti-Apolipoprotein B antibody (ab50069) 使用論文

    ab50069 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunoprecipitation
    Immuno-precipitation step Other - protein G dynabeads
    Sample Human Cell lysate - other (Huh7.5 human hepatoma cell line conditioned medium)
    Specification Huh7.5 human hepatoma cell line conditioned medium
    Total protein in input 5e+006 cells
    Username

    Abcam user community

    Verified customer

    投稿 Dec 16 2013

    Application Western blot
    Loading amount 1e+006 cells
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris, MOPS running buffer)
    Sample Human Cell lysate - whole cell (Huh7.5 human hepatoma cell line)
    Specification Huh7.5 human hepatoma cell line
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Constantin Takacs

    Verified customer

    投稿 Dec 02 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"