製品の概要

  • 製品名Anti-Apolipoprotein B antibody
    Apolipoprotein B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Apolipoprotein B
  • 特異性ab31992 recognizes Apolipoprotein B48 and B100, as these proteins are highly conserved.
  • アプリケーション適用あり: WB, Immunodiffusionmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    SDS PAGE purified mouse Apolipoprotein B48 (very low density lipoprotein) from pooled mouse plasma.

アプリケーション

Our Abpromise guarantee covers the use of ab31992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
Immunodiffusion
  • 追加情報Radial Immunodiffusion (RID): 1/10 - 1/25.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 516 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
    • 関連疾患Hypobetalipoproteinemia, familial, 1
      Familial ligand-defective apolipoprotein B-100
      Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
    • 配列類似性Contains 1 vitellogenin domain.
    • 翻訳後修飾Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
    • 細胞内局在Cytoplasm. Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Apo B-100 antibody
      • Apo B-48 antibody
      • ApoB 100 antibody
      • ApoB 48 antibody
      • APOB antibody
      • APOB_HUMAN antibody
      • Apolipoprotein B 100 antibody
      • Apolipoprotein B antibody
      • Apolipoprotein B-48 antibody
      • Apolipoprotein B48 antibody
      • FLDB antibody
      • LDLCQ4 antibody
      see all

    Anti-Apolipoprotein B antibody (ab31992) 使用論文

    This product has been referenced in:
    • Johnson LA  et al. Diabetic atherosclerosis in APOE*4 mice: synergy between lipoprotein metabolism and vascular inflammation. J Lipid Res 54:386-96 (2013). Mouse . Read more (PubMed: 23204275) »
    • Maczuga P  et al. Embedding siRNA sequences targeting Apolipoprotein B100 in shRNA and miRNA scaffolds results in differential processing and in vivo efficacy. Mol Ther : (2012). Read more (PubMed: 23089734) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (liver, brain)
    Loading amount 15 µg
    Specification liver, brain
    Gel Running Conditions Reduced Denaturing
    Blocking step 5%milk+1%BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 11 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - other (Mouse C57/BL6 EDTA plasma)
    Loading amount 30 µg
    Specification Mouse C57/BL6 EDTA plasma
    Treatment starving O/N
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5%
    Username

    Dr. Andreas Gille

    Verified customer

    投稿 Feb 12 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"