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Full length native protein (purified) corresponding to Mouse Apolipoprotein B. Apolipoprotein B48 purified from pooled mouse plasma very low-density lipoprotein.
Our Abpromise guarantee covers the use of ab20737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 18497885|
|ELISA||Use at an assay dependent concentration. PubMed: 21668821|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Immunodiffusion||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 549 kDa.|
ab20737 staining Apolipoprotein in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% neutral buffered formalin and blocked with 1.5% serum for 45 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (8.5ug/ml in 0.3% Triton X-100 in PBS) for 1 hour at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (7ug/ml) was used as the secondary antibody.
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