Anti-Apolipoprotein A I 抗体 [EP1368Y] (ab52945)

製品の概要

  • 製品名Anti-Apolipoprotein A I antibody [EP1368Y]
    Apolipoprotein A I 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1368Y] to Apolipoprotein A I
  • アプリケーション適用あり: Sandwich ELISA, IHC-FoFr, WB, IP, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human Apolipoprotein A I

  • ポジティブ・コントロール
    • Fetal liver lysate, human liver tissue and HEPG2 cell; Rat astrocytes.
  • 特記事項

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

アプリケーション

Our Abpromise guarantee covers the use of ab52945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA 1/2000 - 1/4000. Can be paired for Sandwich ELISA with Goat polyclonal to Apolipoprotein A I (HRP) (ab20784). PubMed: 20421239
IHC-FoFr Use at an assay dependent concentration.
WB 1/20000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
IP 1/60.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
    • 組織特異性Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
    • 関連疾患Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
      Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I.
      Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed.
      Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
    • 配列類似性Belongs to the apolipoprotein A1/A4/E family.
    • 翻訳後修飾Palmitoylated.
      Phosphorylation sites are present in the extracelllular medium.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Apo-AI antibody
      • ApoA I antibody
      • ApoA-I antibody
      • APOA1 antibody
      • APOA1_HUMAN antibody
      • Apolipoprotein A-I(1-242) antibody
      • Apolipoprotein A1 antibody
      • Apolipoprotein AI antibody
      • Brp14 antibody
      • Ltw1 antibody
      • Lvtw1 antibody
      • Sep1 antibody
      • Sep2 antibody
      see all

    Anti-Apolipoprotein A I antibody [EP1368Y] 画像

    • ab52945 staining Apolipoprotein A I in mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices, permeablized with 0.1 M PBS with 1% Triton X and blocked with 10% serum for 60 minutes at 24°C. The sample was incubated with primary antibody (1/100 in 0.1M PBST with 10% donkeys serum) at 4°C for 24 hours. An Alexa Fluor® 488-conjugated donkey monoclonal (1/1000) was used as the secondary antibody.

    • Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/20000 dilution + fetal liver lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 31 kDa
      Observed band size : 31 kDa
    • Immunocytochemistry/Immunofluorescence analysis rat primary astrocyte culture labelling Apolipoprotein A I with ab52945 at 1/2000. Cells were fixed with methanol and permeabilised using 0.1M PBS with 1% Triton X. An Alexa Fluor® 488-conjugated donkey antibody at 1/3000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

      See Abreview

    • ab52945 at 1/100 dilution staining Apolipoprotein A I in human liver by Immunohistochemistry, Paraffin embedded tissue.
    • ab52945 at 1/100 dilution staining Apolipoprotein A I in HEPG2 cells by Immunofluorescence.

    Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) 使用論文

    This product has been referenced in:
    • Lukkahatai N  et al. Proteomic Serum Profile of Fatigued Men Receiving Localized External Beam Radiation Therapy for Non-Metastatic Prostate Cancer. J Pain Symptom Manage N/A:N/A (2013). Read more (PubMed: 23916682) »
    • Nguyen SD  et al. Spontaneous remodeling of HDL particles at acidic pH enhances their capacity to induce cholesterol efflux from human macrophage foam cells. J Lipid Res 53:2115-25 (2012). Read more (PubMed: 22855736) »

    See all 6 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (Primary astrocyte culture)
    Permeabilization Yes - 0.1M PBS with 1% Triton X
    Specification Primary astrocyte culture
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative Methanol
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    投稿 Sep 06 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Mouse Tissue sections (Brain)
    Permeabilization Yes - 0.1 M PBS with 1% Triton X
    Specification Brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    投稿 Feb 05 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Brain lysate)
    Gel Running Conditions Reduced Denaturing
    Loading amount 30 µg
    Specification Brain lysate
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    投稿 Jan 28 2016

    Thank you for your enquiry and interest in our products.

    Currently, the blocking peptide for this particular antibody is not available commercially. However, if you wish to check the specificity I would advise to run a no-primary (only second...

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    Thank you for bringing this to our attention. I agree that your protocol appears correct and that the antibody may be at fault. We sometimes see an improvement in signal for some antibodies after switching the blocking buffer from 5% milk to BSA, but g...

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    Thank you for contacting us.

    I am sorry that the vial you received was not at the predicted concentration and I apologize for the inconvenience. I have issued a free of charge replacement vial with the order number of xxxxxx.


    Ple...

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    Thank you for contacting us. Yes, the Apolipoprotein A I protein ab90760 is the correct target for the antibodies ab52945 and ab20918. However, please note that the only data we have for sELISA are : - ab52945 has been used in sELISA in pair wi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"