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RabMAb

Anti-Apolipoprotein A I 抗体 [EP1368Y] (ab52945)

製品の概要

  • 製品名
    Anti-Apolipoprotein A I antibody [EP1368Y]
    Apolipoprotein A I 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1368Y] to Apolipoprotein A I
  • アプリケーション
    適用あり: Sandwich ELISA, IHC-FoFr, WB, IP, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human Apolipoprotein A I

  • ポジティブ・コントロール
    • Fetal liver lysate, human liver tissue and HEPG2 cell
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

アプリケーション

Our Abpromise guarantee covers the use of ab52945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA 1/2000 - 1/4000. Can be paired for Sandwich ELISA with Goat polyclonal to Apolipoprotein A I (HRP) (ab20784). PubMed: 20421239
IHC-FoFr Use at an assay dependent concentration.
WB 1/20000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
IP 1/60.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能
      Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
    • 組織特異性
      Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
    • 関連疾患
      Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
      Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I.
      Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed.
      Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
    • 配列類似性
      Belongs to the apolipoprotein A1/A4/E family.
    • 翻訳後修飾
      Palmitoylated.
      Phosphorylation sites are present in the extracelllular medium.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Apo-AI antibody
      • ApoA I antibody
      • ApoA-I antibody
      • APOA1 antibody
      • APOA1_HUMAN antibody
      • Apolipoprotein A-I(1-242) antibody
      • Apolipoprotein A1 antibody
      • Apolipoprotein AI antibody
      • Brp14 antibody
      • Ltw1 antibody
      • Lvtw1 antibody
      • Sep1 antibody
      • Sep2 antibody
      see all

    画像

    • ab52945 at 1/100 dilution staining Apolipoprotein A I in human liver by Immunohistochemistry, Paraffin embedded tissue.
    • ab52945 at 1/100 dilution staining Apolipoprotein A I in HEPG2 cells by Immunofluorescence.
    • Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/20000 dilution + fetal liver lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 31 kDa
      Observed band size : 31 kDa

    参考文献

    This product has been referenced in:
    • Kim K  et al. C1QBP is upregulated in colon cancer and binds to apolipoprotein A-I. Exp Ther Med 13:2493-2500 (2017). Read more (PubMed: 28565870) »
    • Holzer M  et al. Refined purification strategy for reliable proteomic profiling of HDL2/3: Impact on proteomic complexity. Sci Rep 6:38533 (2016). Read more (PubMed: 27917957) »

    See all 8 Publications for this product

    レビューと Q&A

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.1 M PBS with 1% Triton X
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    投稿 Feb 05 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain lysate)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Brain lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    投稿 Jan 28 2016

    Thank you for your enquiry and interest in our products.

    Currently, the blocking peptide for this particular antibody is not available commercially. However, if you wish to check the specificity I would advise to run a no-primary (only second...

    Read More

    Thank you for bringing this to our attention. I agree that your protocol appears correct and that the antibody may be at fault. We sometimes see an improvement in signal for some antibodies after switching the blocking buffer from 5% milk to BSA, but g...

    Read More

    Thank you for contacting us.

    I am sorry that the vial you received was not at the predicted concentration and I apologize for the inconvenience. I have issued a free of charge replacement vial with the order number of xxxxxx.


    Ple...

    Read More

    Thank you for contacting us. Yes, the Apolipoprotein A I protein ab90760 is the correct target for the antibodies ab52945 and ab20918. However, please note that the only data we have for sELISA are : - ab52945 has been used in sELISA in pair wi...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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