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A synthetic peptide corresponding to residues near the N-terminus of human Apolipoprotein A I
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
Our Abpromise guarantee covers the use of ab52945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||1/2000 - 1/4000. Can be paired for Sandwich ELISA with Goat polyclonal to Apolipoprotein A I (HRP) (ab20784). PubMed: 20421239|
|IHC-FoFr||Use at an assay dependent concentration.|
|WB||1/20000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/100 - 1/250.|
ab52945 staining Apolipoprotein A I in mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices, permeablized with 0.1 M PBS with 1% Triton X and blocked with 10% serum for 60 minutes at 24°C. The sample was incubated with primary antibody (1/100 in 0.1M PBST with 10% donkeys serum) at 4°C for 24 hours. An Alexa Fluor® 488-conjugated donkey monoclonal (1/1000) was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence analysis rat primary astrocyte culture labelling Apolipoprotein A I with ab52945 at 1/2000. Cells were fixed with methanol and permeabilised using 0.1M PBS with 1% Triton X. An Alexa Fluor® 488-conjugated donkey antibody at 1/3000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).
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