製品の概要

  • 製品名Anti-Apolipoprotein A I antibody
    Apolipoprotein A I 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Apolipoprotein A I
  • 特異性Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. This antibody reacts with mouse apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins.
  • アプリケーション適用あり: Sandwich ELISA, ELISA, IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Full length native apoLipoprotein Type A-I (purified).

  • 特記事項This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab7614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA 1/5000 - 1/10000. Can be paired for Sandwich ELISA with Rabbit polyclonal to Apolipoprotein A I (ab20453).
ELISA 1/5000 - 1/10000.
IHC-P 1/250 - 1/500.
WB 1/500 - 1/1000.
IP 1/100.

ターゲット情報

  • 機能Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 組織特異性Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
  • 関連疾患Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
    Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I.
    Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed.
    Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • 配列類似性Belongs to the apolipoprotein A1/A4/E family.
  • 翻訳後修飾Palmitoylated.
    Phosphorylation sites are present in the extracelllular medium.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apo-AI antibody
    • ApoA I antibody
    • ApoA-I antibody
    • APOA1 antibody
    • APOA1_HUMAN antibody
    • Apolipoprotein A-I(1-242) antibody
    • Apolipoprotein A1 antibody
    • Apolipoprotein AI antibody
    • Brp14 antibody
    • Ltw1 antibody
    • Lvtw1 antibody
    • Sep1 antibody
    • Sep2 antibody
    see all

Anti-Apolipoprotein A I antibody 画像

  • ab7614 staining Apolipoprotein A I in mouse liver tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with Proteinase K and then blocking with 2% BSA for 20 minutes was performed. The primary antibody was diluted 1/6000 and incubated with sample for 24 hours at 4°C in 1% BSA. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.

    See Abreview

  • All lanes : Anti-Apolipoprotein A I antibody (ab7614) at 1/5000 dilution

    Lane 1 : Whole tissue lysate prepared from mouse liver
    Lane 2 : Whole tissue lysate prepared from mouse liver
    Lane 3 : Whole tissue lysate prepared from mouse liver

    Lysates/proteins at 50 µg per lane.

    Secondary
    Donkey anti-goat Alexa Fluor® 647 at 1/2500 dilution

    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

    Image courtesy of Hua Jiang by Abreview.

Anti-Apolipoprotein A I antibody (ab7614) 使用論文

This product has been referenced in:
  • Hesse D  et al. Hepatic trans-Golgi action coordinated by the GTPase ARFRP1 is crucial for lipoprotein lipidation and assembly. J Lipid Res 55:41-52 (2014). WB ; Mouse . Read more (PubMed: 24186947) »
  • Aseem O  et al. Cubilin Maintains Blood Levels of HDL and Albumin. J Am Soc Nephrol N/A:N/A (2013). Mouse . Read more (PubMed: 24357674) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 10 µg
Specification Liver
Gel Running Conditions Reduced Non-Denaturing (Native) (4-20 %)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Oct 02 2012

Thank you for contacting us. We currently only have a mouse ELISA kit for Apolipoprotein CIII: ab108811 However, we do have antibodies to each of your targets of interest that can be used in ELISA on mouse samples: Apolipoprotein A I: ab7614 or ab20453...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver, stomach, kidney, testes, lung, heart, etc)
Specification Liver, stomach, kidney, testes, lung, heart, etc
Fixative Formaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2%
Username

Miss. Judit Marsillach

Verified customer

投稿 Jul 24 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Loading amount 50 µg
Specification liver
Gel Running Conditions Reduced Denaturing (9%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Hua Jiang

Verified customer

投稿 Jan 02 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Purified protein (purified protein (BIODESIGN))
Loading amount 0.5 µg
Specification purified protein (BIODESIGN)
Gel Running Conditions Reduced Denaturing
Blocking step 5%mill+1%BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Feb 11 2008

Thank you for your enquiry. This product was tested using a direct ELISA using purified mouse Apo A-1. It was not tested using different concentrations of mouse serum. I am sorry you are experiencing problems with this antibody. Below please...

Read More

Thank you for your enquiry and for taking your time to fill in the on-line Questionnaire. We are very sorry to hear that you are having problem with this antibody. We have searched our database and found that this is a popular selling product and y...

Read More

Thank you for your enquiry. We would suggest performing a regular sandwich ELISA protocol.If you need anything further or any help then please let us know.

Thank you for your enquiry. The immunogen is from mouse that's why the antibody recognizes mouse Apolipoprotein A I.

Thank you for your enquiry. Unfortunately, the purified Apolipoprotein A I protein which has been used is not commercially available.

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"