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Purified mouse apolipoprotein AI from pooled mouse plasma high density lipoprotein.
Our Abpromise guarantee covers the use of ab20453 in the following tested applications.
|ICC/IF||Use a concentration of 5 µg/ml.|
|RID||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 19619511|
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Goat polyclonal to Apolipoprotein A I (ab7614).|
ab20453 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab20453 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.