製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to APOBEC4
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Horse, Dog, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human APOBEC4.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Human Testis; Mouse Testis; Rat Testis; Human Fetal Brain; Human Fetal Lung.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab103737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).

ターゲット情報

画像

  • All lanes : Anti-APOBEC4 antibody (ab103737) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : Testis (Rat) Tissue Lysate
    Lane 4 : Brain (Human) Tissue Lysate - fetal normal tissue (ab29467)
    Lane 5 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

プロトコール

参考文献

ab103737 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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