製品の概要

  • 製品名Anti-APOBEC3G antibody - C-terminal
    APOBEC3G 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to APOBEC3G - C-terminal
  • アプリケーション適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human APOBEC3G (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • A375 cell lysate and human breast carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

ターゲット情報

  • 機能DNA deaminase (cytidine deaminase) that mediates a form of innate resistance to retroviral infections (at least to HIV-1 infection) by triggering G-to-A hypermutation in the newly synthesized viral DNA. The replacements C-to-U in the minus strand DNA of HIV-1 during reverse transcription, leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. Modification of both DNA strands is not excluded. This antiviral activity is neutralized by the virion infectivity factor (VIF), that prevents the incorporation of APOBEC3G into progeny HIV-1 virions by both inhibiting its translation and/or by inducing its ubiquitination and subsequent degradation by the 26S proteasome. APOBEC3G binds a variety of RNAs, but does not display detectable APOB, NF1 and NAT1 mRNA editing.
  • 組織特異性Expressed in spleen, testes, ovary and peripheral blood leukocytes and CD4+ lymphocytes. Also expressed in non-permissive peripheral blood mononuclear cells, and several tumor cell lines; no expression detected in permissive lymphoid and non-lymphoid cell lines.
  • 配列類似性Belongs to the cytidine and deoxycytidylate deaminase family.
  • 翻訳後修飾Ubiquitinated in the presence of HIV-1 VIF. Association with VIF targets the protein for proteolysis by the ubiquitin-dependent proteasome pathway.
  • 細胞内局在Cytoplasm. Nucleus. Mainly cytoplasmic. Small amount are found in the nucleus. During HIV-1 infection, virion-encapsidated in absence of HIV-1 VIF.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A3G antibody
    • ABC3G_HUMAN antibody
    • APOBEC related cytidine deaminase antibody
    • APOBEC related protein antibody
    • APOBEC-related cytidine deaminase antibody
    • APOBEC-related protein 9 antibody
    • APOBEC-related protein antibody
    • APOBEC3G antibody
    • Apolipoprotein B editing enzyme catalytic polypeptide like 3G antibody
    • Apolipoprotein B mRNA editing enzyme catalytic polypeptide 3G antibody
    • Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3G antibody
    • Apolipoprotein B mRNA editing enzyme catalytic subunit 3G antibody
    • apolipoprotein B mRNA editing enzyme cytidine deaminase antibody
    • apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like antibody
    • ARCD antibody
    • ARP-9 antibody
    • ARP9 antibody
    • bK150C2.7 antibody
    • CEM-15 antibody
    • CEM15 antibody
    • deoxycytidine deaminase antibody
    • dJ494G10.1 antibody
    • DNA dC dU editing enzyme APOBEC 3G antibody
    • DNA dC->dU editing enzyme antibody
    • DNA dC->dU-editing enzyme APOBEC-3G antibody
    • EC 3.5.4. antibody
    • FLJ12740 antibody
    • MDS019 antibody
    • OTTHUMP00000028911 antibody
    • phorbolin-like protein antibody
    • phorbolin-like protein MDS019 antibody
    see all

Anti-APOBEC3G antibody - C-terminal 画像

  • Anti-APOBEC3G antibody - C-terminal (ab71634) at 1/100 dilution + A375 cell lysate at 12.5 µg

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Additional bands at : 33 kDa,35 kDa. We are unsure as to the identity of these extra bands.
  • ab71634 at 1/50 dilution, staining APOBEC3G in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-APOBEC3G antibody - C-terminal (ab71634) 使用論文

This product has been referenced in:
  • Pereira NZ  et al. Upregulation of innate antiviral restricting factor expression in the cord blood and decidual tissue of HIV-infected mothers. PLoS One 8:e84917 (2013). WB ; Human . Read more (PubMed: 24367701) »

See 1 Publication for this product

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Thank you for your enquiry. I have searched our catalog for antibodies to APOBEC3G and HIV tat proteins that have been used successfully in ELISA. Here are some recommendations: For APOBEC3G: ab71634 and ab38604, which are both available for €360.00 + ...

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