Anti-APOBEC3G/A3G 抗体 (ab109727)
Key features and details
- Goat polyclonal to APOBEC3G/A3G
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-APOBEC3G/A3G antibody
APOBEC3G/A3G 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to APOBEC3G/A3G -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Cynomolgus monkey -
免疫原
Synthetic peptide corresponding to Human APOBEC3G/A3G aa 350-450 (C terminal).
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ポジティブ・コントロール
- Daudi cell lysate, Human kidney and Human testis tissues
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109727の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).
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IHC-P |
Use a concentration of 2.5 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa). |
IHC-P
Use a concentration of 2.5 µg/ml. |
ターゲット情報
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機能
DNA deaminase (cytidine deaminase) that mediates a form of innate resistance to retroviral infections (at least to HIV-1 infection) by triggering G-to-A hypermutation in the newly synthesized viral DNA. The replacements C-to-U in the minus strand DNA of HIV-1 during reverse transcription, leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. Modification of both DNA strands is not excluded. This antiviral activity is neutralized by the virion infectivity factor (VIF), that prevents the incorporation of APOBEC3G into progeny HIV-1 virions by both inhibiting its translation and/or by inducing its ubiquitination and subsequent degradation by the 26S proteasome. APOBEC3G binds a variety of RNAs, but does not display detectable APOB, NF1 and NAT1 mRNA editing. -
組織特異性
Expressed in spleen, testes, ovary and peripheral blood leukocytes and CD4+ lymphocytes. Also expressed in non-permissive peripheral blood mononuclear cells, and several tumor cell lines; no expression detected in permissive lymphoid and non-lymphoid cell lines. -
配列類似性
Belongs to the cytidine and deoxycytidylate deaminase family. -
翻訳後修飾
Ubiquitinated in the presence of HIV-1 VIF. Association with VIF targets the protein for proteolysis by the ubiquitin-dependent proteasome pathway. -
細胞内局在
Cytoplasm. Nucleus. Mainly cytoplasmic. Small amount are found in the nucleus. During HIV-1 infection, virion-encapsidated in absence of HIV-1 VIF. - Information by UniProt
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参照データベース
- Entrez Gene: 200316 Human
- Entrez Gene: 60489 Human
- Omim: 607113 Human
- SwissProt: Q9HC16 Human
- Unigene: 659809 Human
- Unigene: 660143 Human
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別名
- A3G antibody
- ABC3G_HUMAN antibody
- APOBEC related cytidine deaminase antibody
see all
画像
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Anti-APOBEC3G/A3G antibody (ab109727) at 0.5 µg/ml + Daudi cell lysate (in RIPA buffer) at 35 µg/ml
Developed using the ECL technique.
Predicted band size: 46 kDa
Primary incubation was 1 hour -
ab109727 at a concentration of 2.5 µg/ml staining APOBEC3G/A3G in Human Kidney, Formalin-Fixed, Paraffin-Embedded section.
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ab109727 at a concentration of 2.5 µg/ml staining APOBEC3G/A3G in Human Testis, Formalin-Fixed, Paraffin-Embedded section.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab109727 は 1 報の論文で使用されています。
- Evangelou K et al. Pulmonary infection by SARS-CoV-2 induces senescence accompanied by an inflammatory phenotype in severe COVID-19: possible implications for viral mutagenesis. Eur Respir J 60:N/A (2022). PubMed: 35086840