製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to APOA4
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human APOA4 aa 49-79 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 337

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

ターゲット情報

  • 関連性
    APOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties
  • 細胞内局在
    Secreted
  • 参照データベース
  • 別名
    • Apo AIV antibody
    • APOA 4 antibody
    • ApoA IV antibody
    • APOA4 antibody
    • Apolipoprotein A IV antibody
    • Apolipoprotein A4 antibody
    • MGC142154 antibody
    • MGC142156 antibody
    see all

画像

  • All lanes : Anti-APOA4 antibody (ab72395) at 1/60 dilution

    Lane 1 : NCI-H460 cell lysate
    Lane 2 : NCI-H460 cell lysate with immunising peptide

    Lysates/proteins at 12.5 µg per lane.


    Predicted band size : 45 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab72395 at 1/50 dilution staining APOA4 in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

参考文献

This product has been referenced in:

See 1 Publication for this product

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