Anti-APOA1BP 抗体 (ab75114)
Key features and details
- Rabbit polyclonal to APOA1BP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-APOA1BP antibody
APOA1BP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to APOA1BP -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Extracts from HeLa cells. A549 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75114の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 32 kDa.
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ICC/IF |
1/500 - 1/1000.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 32 kDa. |
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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関連性
APOA1BP interacts with apolipoprotein A-I (apoA-I), the major apolipoprotein of high-density lipoproteins (HDLs). It is secreted into some bodily fluids, and its synthesis and secretion are stimulated in vitro by incubating cells with apoA-I. -
細胞内局在
Mitochondrion. Secreted. Note: In sperm, secretion gradually increases during capacitation -
参照データベース
- Entrez Gene: 128240 Human
- Entrez Gene: 246703 Mouse
- Entrez Gene: 295229 Rat
- Omim: 608862 Human
- SwissProt: Q8NCW5 Human
- SwissProt: Q8K4Z3 Mouse
- SwissProt: B0BNM1 Rat
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別名
- AI BP antibody
- AIBP antibody
- ApoA I binding protein antibody
see all
画像
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All lanes : Anti-APOA1BP antibody (ab75114) at 1/500 dilution
Lane 1 : Extracts from HeLa cells
Lane 2 : Extracts from HeLa cells with immunizing peptide at 5 µg
Lysates/proteins at 20 µg per lane.
Predicted band size: 32 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of APOA1BP in A549 cells, using ab75114, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab75114 は 2 報の論文で使用されています。
- Duan M et al. Mitochondrial apolipoprotein A-I binding protein alleviates atherosclerosis by regulating mitophagy and macrophage polarization. Cell Commun Signal 20:60 (2022). PubMed: 35525979
- Choi SH et al. AIBP augments cholesterol efflux from alveolar macrophages to surfactant and reduces acute lung inflammation. JCI Insight 3:N/A (2018). PubMed: 30135304