製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to APMAP
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide within N terminal amino acids 72-121 (FKEPPLLLGV LHPNTKLRQA ERLFENQLVG PESIAHIGDV MFTGTADGRV) of Human APMAP (NP_065392)

  • ポジティブ・コントロール
    • Fetal liver lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab81176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

ターゲット情報

  • 機能
    Exhibits strong arylesterase activity with beta-naphthyl acetate and phenyl acetate. May play a role in adipocyte differentiation.
  • 組織特異性
    Liver, glomerular and tubular structures of the kidney, endothelial cells, arterial wall and pancreatic islets of Langerhans (at protein level). Found ubiquitously in adult as well as in embryonic tissues. In adult tissue, the highest expression is found in the liver, placenta and heart. Found on the cell surface of monocytes. In embryonic tissue, the highest expression levels is found in the liver and the kidney.
  • 配列類似性
    Belongs to the strictosidine synthase family.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adipocyte plasma membrane associated protein antibody
    • Adipocyte plasma membrane-associated protein antibody
    • apmap antibody
    • APMAP_HUMAN antibody
    • BSCv antibody
    • BSCv protein antibody
    • C20orf3 antibody
    • Chromosome 20 open reading frame 3 antibody
    • Protein BSCv antibody
    see all

画像

  • Anti-APMAP antibody (ab81176) at 1 µg/ml + Fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab81176 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver lysate)
Gel Running Conditions
Reduced Denaturing (NUPAGE 4-12% BT GEL 1.5MM)
Loading amount
20 µg
Specification
Liver lysate
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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投稿 Jul 04 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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