製品の概要

  • 製品名
    Anti-APM2 antibody
  • 製品の詳細
    Rabbit polyclonal to APM2
  • アプリケーション
    適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from C terminal region of human APM2 protein Peptide available as ab95393.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.08% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Ammonium Sulphate Precipitation
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab79579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration. Used succesfully in ELISA using ab95393 APM2 Peptide to create the standard curve.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See the review submitted by anonymous on 21 Jul 2010.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 8 kDa.Can be blocked with Human APM2 peptide (ab95393).

ターゲット情報

  • 関連性
    APM2 gene is exclusively expressed in adipose tissue. Its function is currently unknown. APM2 has been shown to be associated with cisplatin resistance in cell lines. It is not found in cisplatin sensitive cell lines, implicating APM2 in the development of cisplatin resistance in tumor cells.
  • 参照データベース
  • 別名
    • Adipose most abundant gene transcript 2 protein antibody
    • Adipose specific 2 antibody
    • APM 2 antibody
    • C10orf116 antibody
    • Chromosome 10 open reading frame 116 antibody
    • OTTHUMP00000020019 antibody
    • RP11-96C23.4 antibody
    see all

画像

  • ab79579 detecting APM2 in Human serum by indirect ELISA. Samples were blocked with 5% BSA for 1 hour at 26°C and were incubated with the primary antibody (1/1000) for 3 hours at 26°C. An HRP-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. ab95393 (APM2 Peptide) was used for establishing the standard curve.

    See Abreview

参考文献

ab79579 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Serum (Serum)
Specification
Serum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Type
Indirect
Username

Abcam user community

Verified customer

投稿 Sep 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jul 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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