製品の概要

  • 製品名
    Anti-APJ Receptor antibody
    APJ Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to APJ Receptor
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human APJ Receptor.

    (Peptide available as ab101728.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Brain (Human) Tissue Lysate; U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate; HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human liver cancer.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 42 kDa).
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia.
  • 組織特異性
    Widely expressed in the brain, in glial cells, astrocytes and neuronal subpopulations, as well as in the spleen, thymus, ovary, small intestine and colon.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AGTR L1 antibody
    • AGTRL 1 antibody
    • AGTRL1 antibody
    • Angiotensin II receptor like 1 antibody
    • Angiotensin receptor like 1 antibody
    • Angiotensin receptor-like 1 antibody
    • Apelin receptor antibody
    • APJ (apelin) receptor antibody
    • APJ antibody
    • APJ receptor antibody
    • APJ_HUMAN antibody
    • APJR antibody
    • APLNR antibody
    • B78 antibody
    • FLJ90771 antibody
    • G protein coupled receptor APJ antibody
    • G-protein coupled receptor APJ antibody
    • G-protein coupled receptor HG11 antibody
    • GPCR34 antibody
    • HG 11 antibody
    • HG11 antibody
    • HG11 orphan receptor antibody
    • MGC45246 antibody
    • MSR antibody
    • msr/apj antibody
    see all

Anti-APJ Receptor antibody 画像

  • All lanes : Anti-APJ Receptor antibody (ab84296) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • ICC/IF image of ab84296 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84296, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa and HepG2 cells at 5µg/ml.
  • IHC image of APJ Receptor staining in Human liver cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84296, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab84296 staining APJ Receptor in Human endothelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.2% and blocked with 20% serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal(1/300) was used as the secondary antibody.

    See Abreview

Anti-APJ Receptor antibody (ab84296) 使用論文

This product has been referenced in:
  • Chen L  et al. Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis. J Ophthalmol 2015:186946 (2015). WB, IHC-Fr, ICC/IF ; Rat . Read more (PubMed: 26491547) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6.0, 100C 20 minutes
Sample
Human Tissue sections (Liver)
Specification
Liver
Permeabilization
No
Fixative
Formaldehyde
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Abcam user community

Verified customer

投稿 Oct 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (endothelial cells)
Loading amount
5000 cells
Specification
endothelial cells
Gel Running Conditions
Reduced Non-Denaturing (Native)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Mar 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (artery)
Specification
artery
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Mar 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (endothelial cell)
Specification
endothelial cell
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% triton X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Feb 28 2013

Thank you very much for your interest our products. All of the testing data we have is available on the datasheet. So to our knowledge, ab66218 has not been tested in WB and ab84296 has not been tested in rat (although it is predicted to work due to hi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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