製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to APG8A
  • アプリケーション
    適用あり: WB, IHC-Frmore details
  • 種交差性
    交差種: Arabidopsis thaliana
    交差が予測される動物種: Human
  • 免疫原

    Recombinant Arabidopsis thaliana APG8A from E. coli.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Predicted molecular weight: 14 kDa.
IHC-Fr Use at an assay dependent concentration. PubMed: 24463818

ターゲット情報

  • 関連性
    APG8A is involved in cytoplasm to vacuole transport (Cvt) vesicles and autophagosomes formation. It may mediate the delivery of the vesicles and autophagosomes to the vacuole via the microtubule cytoskeleton.
  • 細胞内局在
    Cytoplasmic vesicle › cvt vesicle membrane; Lipid-anchor. Cytoplasmic vesicle › autophagosome membrane; Lipid-anchor. Vacuole membrane; Lipid-anchor. Note= Membrane-associated through a lipid anchor.
  • 参照データベース
    • 別名
      • APG8A (autophagy 8A) antibody
      • AT4G21980 antibody
      • ATG8 antibody
      • ATG8A antibody
      • Autophagy 8A antibody
      • Autophagy related protein 8a antibody
      • Autophagy related ubiquitin like modifier ATG8a antibody
      • F1N20.80 antibody
      • F1N20_80 antibody
      see all

    画像

    • All lanes : Anti-APG8A antibody (ab77003) at 1/500 dilution

      Lane 1 : Arabidopsis whole plant extract
      Lane 2 : Maize whole plant extract

      Secondary
      HRP-conjugated anti-rabbit IgG. at 1/20000 dilution

      Predicted band size : 14 kDa

    参考文献

    This product has been referenced in:
    • Qi H  et al. TRAF Family Proteins Regulate Autophagy Dynamics by Modulating AUTOPHAGY PROTEIN6 Stability in Arabidopsis. Plant Cell 29:890-911 (2017). Read more (PubMed: 28351989) »
    • Chen L  et al. Autophagy contributes to regulation of the hypoxia response during submergence in Arabidopsis thaliana. Autophagy 11:2233-46 (2015). Read more (PubMed: 26566261) »

    See all 6 Publications for this product

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Plants Tissue lysate - other (leaves, roots or whole seedlings)
    Gel Running Conditions
    Non-reduced Denaturing (15% PA running gel)
    Loading amount
    60000 µg
    Treatment
    starvation in presence of 20 micromol L-1 E64 for 48hrs
    Specification
    leaves, roots or whole seedlings
    Blocking step
    Blotto B reagent (Santa Cruz Biotech) in TBS as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 4°C
    Username

    Dr. Olga Voitsekhovskaja

    Verified customer

    投稿 Oct 07 2016

    We have not tested this antibody with Barley samples however as this antibody supposed to react with plant samples hence the possibility of its cross reactivity with Barley is quite high.

    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

    To our knowledge, ab77003 has not been tested inimmunogold EM. Therefore, I can offer a discount off a f...

    Read More

    Thank you very much for contacting us.
    Apparently, Chung and colleagues performed their SDS-PAGE with and without 6 M urea, as reported in their publication (attached). You may find details about this protocol in the Materials and Methods section,...

    Read More

    Thank you for your enquiry. A full length, 6xHis tagged protein was used as the immunogen to create ab77003. I hope this is helpful. Please contact us again if you need any further information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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