遺伝子ノックアウト細胞株 検証済
リコンビナント
RabMAb

Anti-Apg12 抗体 [EPR4800] (ab109491)

製品の概要

  • 製品名
    Anti-Apg12 antibody [EPR4800]
    Apg12 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4800] to Apg12
  • アプリケーション
    適用あり: ICC, WB, IHC-Pmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to residues on the C-terminus in Human Apg12.

  • ポジティブ・コントロール
    • Human fetal kidney, HepG2, and Raji lysates; Apg12 transfected 293T lysates; Human breast carcinoma tissue.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/250 - 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    Anti-Apg12 antibody [EPR4800] 画像



    • Predicted band size : 15 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Apg12 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: HCT116 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab109491 observed at 55 kDa. Observed band shows Apg12-ATG5 conjugation. Red - loading control, ab8245, observed at 37 kDa.

      Ab109491 was shown to specifically react with Apg12 in wild-type cells along with additional cross-reactive bands. The band was not seen in Apg12 knockout HAP1 cells. Wild-type and Apg12 knockout samples were subjected to SDS-PAGE.  Ab109491 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Apg12 antibody [EPR4800] (ab109491) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : HepG2 lysate
      Lane 3 : Raji lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 15 kDa
      Observed band shows Apg12-ATG5 conjugation
    • All lanes : Anti-Apg12 antibody [EPR4800] (ab109491) at 1/1000 dilution

      Lane 1 : Apg12 transfected 293T lysate
      Lane 2 : Non-transfected 293T lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 15 kDa
    • ab109491, at 1/250 dilution, staining Apg12 in paraffin-embedded Human breast carcinoma by Immunohistochemistry.

    Anti-Apg12 antibody [EPR4800] (ab109491) 使用論文

    ab109491 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab109491.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録