製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Apg12
  • 由来種
    Rabbit
  • 特異性
    ab155589 detects free Apg12 and Apg12 conjugated to Atg5.
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 1-187 of Human Apg12.

  • ポジティブ・コントロール
    • 293T, A431, NIH3T3 and Rat brain whole cell lysates; Human U87 xenograft tissue; HeLa cells untreated or treated with Tunicamycin.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab155589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 15 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

ターゲット情報

画像

  • Anti-Apg12 antibody (ab155589) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

    Predicted band size: 15 kDa



    12% SDS PAGE
  • Anti-Apg12 antibody (ab155589) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg

    Predicted band size: 15 kDa



    12% SDS PAGE
  • All lanes : Anti-Apg12 antibody (ab155589) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 15 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded U87 xenograft tissue labeling Apg12 with ab155589 at 1/500 dilution.
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Apg12 with ab155589 at 1/500 dilution. Lower panels co-stained with Hoechst 33342.
    Cells were fixed in 4% paraformaldehyde for 15 minutes.

参考文献

This product has been referenced in:
  • Maueröder C  et al. Capability of Neutrophils to Form NETs Is Not Directly Influenced by a CMA-Targeting Peptide. Front Immunol 8:16 (2017). Read more (PubMed: 28191006) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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