製品の概要

  • 製品名Anti-Apelin antibody
    Apelin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Apelin
  • 特異性This antibody reacts specifically with 8,5 kDa of human APLN protein.
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human, Monkey
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide derived from C terminal region of human Apelin

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 9 kDa.
IHC-P 1/500.

ターゲット情報

  • 機能Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior.
  • 組織特異性Expressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain. Secreted by the mammary gland into the colostrum and the milk.
  • 配列類似性Belongs to the apelin family.
  • 翻訳後修飾Several active peptides may be produced by proteolytic processing of the peptide precursor.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AGTRL1 ligand antibody
    • APEL antibody
    • APEL_HUMAN antibody
    • Apelin-13 antibody
    • APJ endogenous ligand antibody
    • Apln antibody
    • XNPEP2 antibody
    see all

Anti-Apelin antibody 画像

  • ab59469 (1:500) staining Apelin in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic/secreted regions of cells in the basal uterine glands.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-Apelin antibody (ab59469) 使用論文

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