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Full length native protein (purified) (Human).
Our Abpromise guarantee covers the use of ab194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||1/2000. Predicted molecular weight: 35.6 kDa.|
|ICC||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration.|
ab194 staining cultured human HeLa cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C. A Cy3® conjugated donkey anti-mouse antibody diluted was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"