製品の概要

  • 製品名
    Anti-Apc6 (phospho T580) antibody
    Apc6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Apc6 (phospho T580)
  • 由来種
    Rabbit
  • 特異性
    ab12282 is specific for the phosphorylated form of APC6 at the T580 residue. MInimal reactivity is expected with the non-phosphorylated form of the protein.
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide: CG-ETGLTpPLETS conjugated to KLH, corresponding to Internal sequence amino acids 575-588 of Human Apc6.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab12282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
  • 追加情報
    IF: Use at an assay dependent dilution.
    WB: 1/500 - 1/3000. Expect bands of approximately 72kDa, 71.5kDa or 65.9 kDa depending on the isoform (predicted molecular weight: 72 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
    • パスウェイ
      Protein modification; protein ubiquitination.
    • 配列類似性
      Belongs to the APC6/CDC16 family.
      Contains 7 TPR repeats.
    • 翻訳後修飾
      Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.
    • 細胞内局在
      Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ANAPC6 antibody
      • Anaphase promoting complex subunit 6 antibody
      • Anaphase-promoting complex subunit 6 antibody
      • Apc 6 antibody
      • APC6 antibody
      • CDC 16 antibody
      • CDC16 (cell division cycle 16 S. cerevisiae homolog) antibody
      • Cdc16 antibody
      • CDC16 homolog antibody
      • CDC16 protein antibody
      • CDC16_HUMAN antibody
      • CDC16Hs antibody
      • Cell division cycle 16 antibody
      • Cell division cycle 16 homolog antibody
      • Cell division cycle protein 16 homolog antibody
      • CUT9 antibody
      • Cyclosome subunit 6 antibody
      see all

    画像

    • All lanes : Anti-Apc6 (phospho T580) antibody (ab12282) at 1/1000 dilution

      Lane 1 : Nocodazole treated HeLa whole cell lysate
      Lane 2 : Asynchronous HeLa whole cell lysate

      Lysates/proteins at 35 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 72 kDa
      Observed band size: 72 kDa



      The blot was probed overnight at 4°C with 1/1000 dilution of ab12282.

    参考文献

    ab12282 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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