製品の概要

  • 製品名Anti-Apc6 antibody
    Apc6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Apc6
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Guinea pig, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Opossum
  • 免疫原

    Synthetic peptide corresponding to a region between residues 175 and 225 of human Apc6, NP_003894.3.

  • ポジティブ・コントロール
    • Whole cell lysate from Hela and NIH3T3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 72, 90 kDa (predicted molecular weight: 72 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the APC6/CDC16 family.
    Contains 7 TPR repeats.
  • 翻訳後修飾Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.
  • 細胞内局在Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANAPC6 antibody
    • Anaphase promoting complex subunit 6 antibody
    • Anaphase-promoting complex subunit 6 antibody
    • Apc 6 antibody
    • APC6 antibody
    • CDC 16 antibody
    • CDC16 (cell division cycle 16 S. cerevisiae homolog) antibody
    • Cdc16 antibody
    • CDC16 homolog antibody
    • CDC16 protein antibody
    • CDC16_HUMAN antibody
    • CDC16Hs antibody
    • Cell division cycle 16 antibody
    • Cell division cycle 16 homolog antibody
    • Cell division cycle protein 16 homolog antibody
    • CUT9 antibody
    • Cyclosome subunit 6 antibody
    see all

Anti-Apc6 antibody 画像

  • All lanes : Anti-Apc6 antibody (ab72136) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 15 µg
    Lane 2 : Whole cell lysate from Hela cells at 5 µg
    Lane 3 : Whole cell lysate from Hela cells at 1.5 µg
    Lane 4 : Whole cell lysate from Hela cells at 0.5 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg


    Predicted band size : 72 kDa
    Observed band size : 72 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation/ Western Blot of Apc6.
    Lane 1: ab72136 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.

    ab72136 at 0.1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Detection:Chemiluminescence with an exposure time of 30 seconds.

Anti-Apc6 antibody (ab72136) 使用論文

ab72136 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab72136.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"